Goat anti Human TNF alpha antibody AHP1212 bio-rad,Goat anti Human TNF alpha antibody

2024-10-22

Goat anti Human TNF alpha antibody

货号:AHP1212

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:TNF alpha

物种:人

宿主:山羊

抗体亚型:IgG

荧光染料:Purified

产品介绍
Goat anti Human TNF alpha antibodyrecognizes human Tumour Necrosis Factor alpha (TNF–alpha), a 17.4kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-kappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. TNF-alpha also plays a role in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
产品详情
Target SpeciesHuman
Product FormPurified IgG - lyophilized
ReconstitutionReconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum PreparationAntisera to human TNFα were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer SolutionPhosphate buffered saline
Preservative StabilisersNone present.
ImmunogenRecombinant human TNFα
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml after reconstitution
RegulatoryFor research purposes only
Guarantee6 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.251.0ug/ml
Western Blotting0.10.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Rabbit anti Goat IgG (Fc):FITCSTAR122FF100 Tests
Rabbit anti Goat IgG (Fc):HRPSTAR122PC,E,WB100 Tests
文献
1.van Horssen, R.et al. (2006) TNF-α in cancer treatment: Molecular insights, antitumor effects, and clinical utility.Oncologist. 11: 397-408.

技术参数

产品应用 elisa;wb

产品类型 Polyclonal Antibody

推荐用于 elisa;wb

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