Bio-Rad AHP1064 GAPDH,Goat anti Human GAPDH antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP1064 GAPDH,Goat anti Human GAPDH antibody

2024-10-22

Goat anti Human GAPDH antibody

货号：AHP1064

规格：0.1 mg

价格：5083

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：GAPDH

物种：人

宿主：山羊

抗体亚型：IgG

荧光染料：Purified

产品介绍
Goat anti Human GAPDH antibodyrecognizes an epitope within the C-terminal (CT) region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.GAPDH translocates to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death (Dastooret al. 2001). GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Burkeet al.1996).
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Dog	√
Mouse	√
Pig	√
Rat	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Antiserum Preparation	Antisera to human GAPDH (CT) were raised by repeatedimmunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution	TRIS buffered saline
Preservative Stabilisers	0.02%Sodium Azide (NaN₃) 0.5%Bovine Serum Albumin
Approx. Protein Concentrations	IgG concentration 0.5mg/ml
Immunogen	Peptide sequence C-HQVVSSDFNSDT corresponding to the C-terminal region of GAPDH (NP_002037.2).
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√		1/2000
Immunohistology - Paraffin¹	√		0.3ug/ml
Western Blotting	√	0.01	0.03ug/ml
¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Histology Positive Control Tissue Human tonsil Western Blotting AHP1064 detects a band of approximately 35kDa in human tonsil, mouse spleen and HeLa cell lysates.
二抗相关产品
Description	Product Code	Applications	Pack Size
Rabbit anti Goat IgG (Fc):FITC	STAR122F	Flow	1 mg
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C;E;WB	1 mg
数据
Western blotting analysis of mouse liver (A) and rat brain (B) lysate (35μg protein in RIPA buffer) using Goat anti Human GAPDH antibody (AHP1064) at 0.5μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
文献
1. Hara, M.R.et al.(2006) GAPDH as a sensor of NO stress.Biochim Biophys Acta. 1762 (5): 502-9.2. Mazzola, J.L. & Sirover, M.A. (2001) Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer's disease and in Huntington's disease fibroblasts.J Neurochem. 76 (2): 442-9.3. Wang Het al.(2013) Hypoxic preconditioning alleviates ethanol neurotoxicity: the involvement of autophagy.Neurotox Res. 24 (4): 472-7.

技术参数

产品应用 ELISA;IHC(P)*;WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;IHC(P)*;WB

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Bio-Rad AHP1064 GAPDH,Goat anti Human GAPDH antibody

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