产品介绍 |
| Rabbit anti Human ATG7 (C-Terminal) antibodyrecognizes human Autophagy-related protein 7, known also as Ubitiquin Activating Enzyme-E1 like Protein, ATG7, and APG7. ATG7 is a ~78 kDa cytoplasmic protein.In mammalian cells, ATG7 is essential for autophagy conjugation systems, autophagosome formation, and starvation-induced bulk degradation of proteins and organelles. Caspase-8 may alter ATG7 levels and thus the ATG7 program of autophagic cell death (Yuet al.2004).ATG7 is evolutionarily conserved exhibiting high homology within mammalian species. It expressed in wide range of tissues including kidney, liver, bone marrow and lymph nodes. |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target SpeciesCross Reactivity |
| Mouse √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to human ATG7 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | Pack Size: 0.1 mg |
| 0.02% Sodium Azide (NaN3) |
| Pack Size: 50 µg |
| <0.1% Sodium Azide (NaN3) |
Immunogen | A peptide corresponding to an 17 amino acid sequence from near the carboxy terminus of human ATG7. |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Frozen | √ | | 10ug/ml |
Western Blotting | √ | 1 | 2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1651 detects a band of approximately 78kDa in Caco-2 cell lysate.
|
可用二抗 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Description | Product Code | Applications | Pack Size |
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
文献 |
| 1.Pampliega, O.et al.(2013) Functional interaction between autophagy and ciliogenesis.Nature. 502 (7470): 194-200.2.Koerver, L.et al.(2019) The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage.EMBO Rep. 20 (10): e48014. |
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