Bio-Rad AAI19AB IgM,Sheep anti Bovine IgM:Alk. Phos.-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AAI19AB IgM,Sheep anti Bovine IgM:Alk. Phos.

2024-10-22

Sheep anti Bovine IgM:Alk. Phos.

货号：AAI19AB

规格：0.5 mg

价格：2635

产品类型：荧光二抗

品牌：Bio-Rad

抗原： IgM

物种：Bovine

宿主：Sheep

抗体亚型：IgG

荧光染料：Alkaline Phosphatase

产品介绍
Sheep anti bovine IgMrecognizes bovine IgM and shows no cross-reactivity with other bovine immunoglobulin classes in immunoelectrophoresis. Sheep anti bovine IgM may cross-react with IgM from other species.
产品详情
Target Species	Bovine
Product Form	Purified IgG conjugated to Alkaline Phosphatase - liquid
Antiserum Preparation	Antisera to bovine IgM were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
BufferSolution	50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
Preservative Stabilisers	0.09% Sodium Azide 0.2% Bovine Serum Albumin
Immunogen	Purified bovine IgM.
Approx. Protein Concentrations	IgG concentration 0.5 mg/ml
Regulatory	For research purposes only
Guarantee	12 monthsfrom dateof despatch
存储条件
Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	1/1000	1/10000
Western Blotting	√	1/5000	1/50000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
文献
1. Nebl, T.et al.(2002) Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes.J Biol Chem. 277 (45): 43399-409.2. Assad, A.et al.(2012) Immunophenotyping and characterization of BNP colostra revealed pathogenic alloantibodies of IgG1 subclass with specifity to platelets, granulocytes and monocytes of all maturation stages.Vet Immunol Immunopathol. 147: 25-34.3. Hamsten, C.et al.(2009) Recombinant surface proteomics as a tool to analyze humoral immune responses in bovines infected byMycoplasma mycoidessubsp.mycoidessmall colony type.Mol Cell Proteomics. 8: 2544-54.4. Mansilla FCet al.(2015) Safety and immunogenicity of a soluble nativeNeospora caninumtachyzoite-extract vaccine formulated with a soy lecithin/β-glucan adjuvant in pregnant cattle.Vet Immunol Immunopathol. 165 (1-2): 75-80.5. Hossain, M.M.et al.(2016) Multiplex Detection of IgG and IgM to Rift Valley Fever Virus Nucleoprotein, Nonstructural Proteins, and Glycoprotein in Ovine and Bovine.Vector Borne Zoonotic Dis. Jul 5. [Epub ahead of print]6. Van Meulder, F.et al.(2015) Analysis of the protective immune response following intramuscular vaccination of calves against the intestinal parasiteCooperia oncophora.Int J Parasitol. 45 (9-10): 637-46.7. Jankowska, A.et al.(2016) Humoral and cellular immune response toHistophilus somnirecombinant heat shock protein 60 kDa in farm animalsVeterinární Medicína. 60 (No. 11): 603-12.8. Çokçalışkan, C.et al.(2019) Effect of simultaneous administration of foot-and-mouth disease (FMD) and anthrax vaccines on antibody response to FMD in sheep.Clin Exp Vaccine Res. 8 (2): 103-9.9. Springer, A.et al. (2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine LungwormDictyocaulus viviparus..Pathogens 2022, 11, 55.10. Di Giacomo, Set al.(2022) Assessment on Different Vaccine Formulation Parameters in the Protection against Heterologous Challenge with FMDV in CattleViruses. 14 (8): 1781.

技术参数

产品应用 E;WB

产品类型 Polyclonal Antibody

推荐用于 E;WB

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Bio-Rad AAI19AB IgM,Sheep anti Bovine IgM:Alk. Phos.

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