Bio-Rad Leptin抗体 AHP1616,Rabbit anti Human Leptin-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad Leptin抗体 AHP1616,Rabbit anti Human Leptin

2024-10-22

Rabbit anti Human Leptin

货号：AHP1616

规格：0.1 mg

价格：4063

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：Leptin

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Purified

产品介绍
Rabbit anti Human Leptin antibodyrecognizes human leptin, a ~16 kDa major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Reconstitution	Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
AntiserumPreparation	Antiserum to human leptin was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	None present
Carrier Free	Yes
Immunogen	Recombinant human leptin(PHP198)
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml after reconstitution
Regulatory	For research purposes only
Guarantee	6 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	0.5	2.0ug/ml
Immunohistology -Paraffin¹	√		0.5ug/ml
Western Blotting	√	0.1	0.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. ELISA This purified human leptin antibody may be used in an ELISA withrecombinant human leptin(PHP198) as the standard. Western Blotting This purified human leptin antibody may be used in Western Blotting applications under either reducing or non-reducing conditions withrecombinant human leptin(PHP198) as the positive control.
可用二抗
Description	Product Code	Applications	Pack Size
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml
相关产品
Description	Product Code	Applications	Pack Size
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml
文献
1.Paracchini, V.et al.(2005) Genetics of leptin and obesity: a HuGE review.Am J Epidemiol. 162 (2): 101-14.

技术参数

产品应用 E; P*; WB

产品类型 Polyclonal Antibody

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Bio-Rad Leptin抗体 AHP1616,Rabbit anti Human Leptin

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