Bio-Rad IFN Gamma 抗体 5552-9009,Mouse anti Human Cytokeratin 19-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad IFN Gamma 抗体 5552-9009,Mouse anti Human Cytokeratin 19

2024-10-22

Mouse anti Human Cytokeratin 19

货号：5552-9009

规格：0.2 mg

价格：3043

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：Cytokeratin 19

物种：人

宿主：小鼠

抗体亚型：IgG2a

荧光染料：Purified

产品介绍
Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2recognizes the rod domain (aa 312-335) of human cytokeratin 19 (Böttger;et al.1995) also known as keratin 19 encoded by the KRT19 gene. Cytokeratin 19 is a 400 amino acid intermediate filament protein lacking a C-terminal tail domain, in contrast to all other intermediate filament proteins.Cytokeratin 19 expression is observed in striated muscle where it is involved in forming the association between the contractile apparatus and dystrophin (Stoneet al.2005). Expression is also seen in many ductal and glandular cells together with a restricted set of normal and neoplasic epithelial cells.Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2 detects a band of ~45 kDa in western blotting using an A-549 human alveolar adenocarcinoma cell line lysate.
产品详情
Target Species	Human
Western Blotting	In Western blotting 40 kD and 19 kD bands are observed.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09%Sodium Azide (NaN₃)
Carrier Free	Yes
Immunogen	Human breast cancer cell lineMCF-7.
Approx. Protein Concentrations	1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
Acknowledgements	PrecisionAb is a trademark of Bio-Rad Laboratories
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
Immunofluorescence	√
Immunohistology-Paraffin¹	√	1/100	1/200
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. ¹This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Clickhereto learn how we validate our PrecisionAb range.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. Histology Positive Control Tissue Appendix
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文献
1.Karsten, U.et al.(1985) Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion.Eur J Cancer Clin Oncol. 21 (6): 733-40.2.Kasper, M.et al.(1987) Histological evaluation of three new monoclonal anti-cytokeratin antibodies. 1. Normal tissues.Eur J Cancer Clin Oncol. 23 (2): 137-47.3.Goletz, S.et al.(1997) Novel alphaGalNAc containing glycans on cytokeratins are recognized invitro by galectins with type II carbohydrate recognition domains.J Cell Sci. 110 ( Pt 14): 1585-96.4.de Neergaard, M.et al.(2010) Epithelial-stromal interaction 1 (EPSTI1) substitutes for peritumoral fibroblasts in the tumor microenvironment.Am J Pathol. 176: 1229-40.5.Ehlicke, F.et al.(2010) Intervertebral disc regeneration: influence of growth factors on differentiation of human mesenchymal stem cells (hMSC).Int J Artif Organs. 33 (4): 244-52.6.Johnston, R.L.et al.(2016) High content screening application for cell-type specific behaviour in heterogeneous primary breast epithelial subpopulations.Breast Cancer Res. 18 (1): 18.7.Orafa, Z.et al. (2022) Quantitative CK19 biomarker detection in breast cancer cell linesJ Med and Life 15 (2): 188-95.

技术参数

产品应用 E; IF; P*; WB

产品类型 Monoclonal Antibody

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Bio-Rad IFN Gamma 抗体 5552-9009,Mouse anti Human Cytokeratin 19

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