Bio-Rad 2150-0555 Collagen III,Native Bovine Collagen III

2024-10-22

Native Bovine Collagen III

货号:2150-0555

规格:10 mg

价格:1420

产品类型:蛋白

品牌:Bio-Rad

产品介绍
Purified collagen type III from bovine skin. Collagen type III occurs in most soft connective tissues. It is composed of trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines.
产品详情

Target Species

Bovine

Product Form

Purified Protein - lyophilized

Preparation

Isolation steps included extraction into dilute acetic acid after pepsin treatment, followed by differential salt precipitation and purification by ion-exchange chromatography.

Preservative Stabilisers

None present

Reconstitution

Reconstitute with 2.0 ml of 0.1M acetic acid, pH 3.0. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Approx. Protein Concentrations

Total protein concentration 5.0 mg/ml after reconstitution

Purity

Non-collagenous proteins are less than 0.5%. Typical contaminants are cross-linked collagen type II dimers and trimers (approximately 10%). Other collagen types constitute less than 5% by immunoassay and analytical chromatography.

Regulatory

For research purposes only

Guarantee

Guaranteed until date of expiry. Please see product label.

Protein Molecular Weight

300 kDa
存储条件
Prior to reconstitution store at +4℃After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted.Avoid repeated freezing and thawing as this may denature the antibody.Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
文献

1. Hitsumoto, Y.et al.(2013) Adhesive properties ofClostridium perfringensto extracellular matrix proteins collagens and fibronectin.Anaerobe. pii: S1075-9964(13)00187-X.

技术参数

产品应用 ELISA

产品类型 Purified Protein

推荐用于 ELISA

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://gibcohyclone.cn/thread-43488.htm
产品询价需求提交
返回
扫码添加客服微信,咨询产品报价