Mouse anti Bovine CD205:FITC BIO-RAD MCA1651F Mouse anti Bovine CD205:FITC

2024-10-29

Mouse anti Bovine CD205:FITC

货号:MCA1651F

规格:0.1 mg

市场价格:3851

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD205

物种:Bovine

宿主:小鼠

抗体亚型:IgG2b

克隆号:CC98

荧光染料:FITC

产品介绍
Mouse anti Bovine CD205 antibody, clone CC98recognizes the bovine CD205 cell surface antigen, a ~210-220 kDa molecule expressed by T cells that are CD2+ve but not WC1+ve. CD205 is also expressed by B cells, and weakly stains B cell follicles.Bovine CD205 has previously been described as the WC6 antigen (Gliddonet al.2004).Dendritic cells (veiled cells) in afferent lymph are strong expressors of CD205 as are dendritic cells in various other tissues.
产品详情
Target SpeciesBovine
Product FormPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer SolutionPhosphate buffered saline
ImmunogenBovine thymocytes
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Approx. Protein ConcentrationsIgG concentration 0.1 mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525
Fusion PartnersSpleen cells from immunised BALB/c micewere fused with cells of the NS1 myeloma cell line
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).Vet Immunol Immunopathol. 39 (1-3): 25-47.2.Howard, C.J.et al.(1996) Afferent lymph veiled cells stimulate proliferative responses in allogeneic CD4+ and CD8+ T cells but not gamma delta TCR+ T cells.Immunology. 88 (4): 558-64.3.Naessens, J.et al.(1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.Vet Immunol Immunopathol. 39 (1-3): 283-90.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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