货号：76064

规格：For 20 vesicle preps

价格：7600

产品类型：外泌体分离

品牌：QIAGEN

Product Details

The exoEasy Maxi Kit uses membrane affinity spin columns to efficiently isolate exosomes and other extracellular vesicles from serum, plasma, cell culture supernatant and other biological fluids. The maxi column format allows the use of large sample volumes, including up to 4 ml plasma or serum or up to 32 ml cell culture supernatant. The procedure is very fast, consistent and highly suited for isolation of EVs for functional analysis of exosomes and other extracellular vesicles.

Performance

The exoEasy protocol for purification of exosomes and other extracellular vesicles is not only faster than ultracentrifugation, but also yields a cleaner preparation. Scanning electron microscopy shows that while both techniques deliver intact vesicles of the expected size, the preparation from ultracentrifugation also contains many smaller structures that do not match the expected size or shape for EVs

Fully intact, spherical vesicles of different sizes between approximately 50-300 nm diameter can also be observed using cryo-electron microscopy.

Features

· Isolate intact exosomes and other extracellular vesicles

·Suitable for functional testing and physical and biochemical analysis

·Purify exosomes and other extracellular vesicles in 25 minutes

·Process multiple samples in parallel using a simple spin-column procedure

·Use with up to 4 ml of plasma or serum or 32 ml of cell culture supernatant

·Suitable for MISEV-compliant protein characterization