mouse anti human AGGRECAN 伯乐MCA1451G AGGRECAN抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti human AGGRECAN 伯乐MCA1451G AGGRECAN抗体

2024-10-29

mouse anti human AGGRECAN

货号：MCA1451G

规格：0.2 mg

市场价格：3043

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：AGGRECAN

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号： 7E1

荧光染料：Purified

产品介绍
Mouse anti Human aggrecan antibody, clone 7E1recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).A panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. There is a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintinoet al.2009).Mouse anti Human aggrecan antibody, clone 7E1 recognizes a ~205 kDa trypsin derived fragment of aggrecan.Note:Originally described as being of the isotype IgM, clone 7E1 was found to be unstable and was re-cloned by the originator and the new stable sub-clone was confirmed as being an IgG1.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Bovine	√
Chicken	X
Fish	X
Rat	X
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Immunogen	Purified human articular cartilage aggrecan.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
Immunocytochemistry	√
Immunohistology - Frozen	√
Immunoprecipitation	√
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
文献
1. Virgintino, D.et al.(2009) Differential distribution of aggrecan isoforms in perineuronal nets of the human cerebral cortex.J Cell Mol Med. 13 (9B): 3151-73.2. Aigner, T.et al.(2002) Prognostic relevance of cell biologic and biochemical features in conventional chondrosarcomas.Cancer. 94: 2273-81.3. Hashemi Beni, B.et al.(2008) Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-β3 in Pellet Culture SystemIranian J basic Med Res. 11: 10-17

技术参数

产品应用 IHC;ELISA;WB;IP;IC

产品类型 Monoclonal Antibody

推荐用于 IHC;ELISA;WB;IP;IC

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mouse anti human AGGRECAN 伯乐MCA1451G AGGRECAN抗体

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