c-Myc Monoclonal Antibody (9E10.3)

货号： MA5-12080

规格：500 µL

价格：4786

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号： 9E10.3

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.2 mg/mL	用法：	1:50-1:200(WB)；1:25-1:50(IHC (P))；1:100(ICC/IF)

产品详细信息MA5-12080 detects-myc in Human samples Does not react with mouse, rat and chicken. Others not tested..MA5-12080 targets c-myc in FACS, IF, IHC (P), IM, IP, and WB applications.The MA5-12080 immunogen is a synthetic peptide, aa 408-439, (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc.靶标信息The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.

数据

c-Myc Antibody (MA5-12080) in ICC/IFImmunofluorescent analysis of Myc tag was performed using 70% confluent His-H3-Myc transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Myc Tag Monoclonal Antibody (Product # MA5-12080) at 1:100 dilution and Histone H3 antibody (Product # 711055) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight, and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Product # A32728) and Goat anti-Rabbit IgG Alexa Fluor Plus 488 (Product # A32731) respectively at a dilution of 1:2000 for 45 minutes at room temperature. Panel a (Nuclei: Red) represents Myc tag. Panel b (Nuclei: Green) represents Histone H3. Panel c (Nuclei: Blue) represents ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel d represents the merged image showing the co-localization of nuclear signals in transfected cells. Panel e represents un-transfected HEK-293 cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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参考文献：

1.American journal of human geneticsMutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis."MA5-12080 was used in western blot to identify MAPKBP1 mutations as a genetic cause of juvenile or late-onset and cilia-independent nephronophthisis"2.OncotargetYWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells: new insights about YWHAE role in the tumor development and metastasis process."Published figure using c-Myc monoclonal antibody (Product # MA5-12080) in Western Blot"3.The Journal of pathologyMYC expression and distribution in normal mature lymphoid cells."MA5-12080 was used in western blot to study which normal mature lymphoid cells express myc"4.Cancer geneticsBCL2, BCL6, IGH, TP53, and MYC protein expression and gene rearrangements as prognostic markers in diffuse large B-cell lymphoma: a study of 44 Turkish patients."MA5-12080 was used in immunohistochemistry to study the prognostic value of immunohistochemical and FISH analysis of BCL2, BCL6, IGH, TP53, and MYC in Turkish patients with diffuse large B-cell lymphoma"5.Journal of cutaneous pathologyEGFR and MYC gene copy number aberrations are more common in squamous cell carcinoma than keratoacanthoma: a FISH study."MA5-12080 was used in immunohistochemistry to use FISH to study the higher frequency of EGFR and MYC gene amplification in squamous cell carcinoma than in keratoacanthoma"

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