HLA-ABC Monoclonal Antibody (W6/32), PE, eBioscience™

货号：12-9983-41,12-9983-42

规格：25 tests,100 tests

价格：957,2339

产品类型：流式抗体

品牌：eBioscience

抗原：HLA-ABC

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号： W6/32

荧光染料：PE

类型:	单抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test(Flow)

产品详细信息Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey.Applications Reported: The W6/32 antibody has been reported for use in flow cytometric analysis.Applications Tested: This W6/32 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.仅用于科研。不用于诊断过程。

数据

HLA-ABC Antibody (12-9983-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (blue histogram) or Anti-Human HLA-ABC PE (purple histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Cell host & microbeCowpox virus inhibits the transporter associated with antigen processing to evade T cell recognition."12-9983 was used in Flow cytometry/Cell sorting to identify cowpox virus proteins that mediate T cell evasion in multiple hosts."AuthorsAlzhanova D,Edwards DM,Hammarlund E,Scholz IG,Horst D,Wagner MJ,Upton C,Wiertz EJ,Slifka MK,Früh KYear2009SpeciesHumanMouse 2. Cellular & molecular immunologyConstruction of soluble Mamu-b*1703, a class I major histocompatibility complex of Chinese rhesus macaques, monomer and tetramer loaded with a simian immunodeficiency virus peptide."12-9983 was used in an ELISA assay to investigate how some antigenic peptides reported in Indian-origin macaques could be directly recruited as ligands for construction of Chinese macaque MHC tetramers."AuthorsOuyang D,Wang X,He X,Xu L,Shi H,Gao Q,Guo HYear2009SpeciesRhesus monkey 3. Clinical and experimental immunologySensitization of human breast cancer cells to natural killer cell-mediated cytotoxicity by proteasome inhibition."12-9983 was used in Flow cytometry/Cell sorting to investigate whether bortezomib can sensitise human breast cancer cells to killing by the human NK cell line, NK-92."AuthorsAmes E,Hallett WH,Murphy WJYear2009SpeciesHuman 4. The Journal of biological chemistrySignaling of a varicelloviral factor across the endoplasmic reticulum membrane induces destruction of the peptide-loading complex and immune evasion."12-9983 was used in Flow cytometry/Cell sorting to show that a small viral factor and their signalling across endoplasmic reticulum membranes are essential for targeted degradation of multi-subunit membrane complexes."AuthorsLoch S,Klauschies F,Schölz C,Verweij MC,Wiertz EJ,Koch J,Tampé RYear2008SpeciesHuman 5. The Journal of biological chemistryIdentification of a lysosomal peptide transport system induced during dendritic cell development."12-9983 was used in Flow cytometry/Cell sorting to suggest a mechanism by which antigenic peptides have access to the lysosomal compartment in professional antigen-presenting cells."

技术参数

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