SAV1 Recombinant Rabbit Monoclonal Antibody (6H5L16)

货号：703002

规格：100 µg

价格：4643

产品类型：抗体和染料

品牌：Thermo Fisher

物种：人

宿主：兔

抗体亚型：IgG

克隆号： 6H5L16

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:100 (ICC/IF)

产品详细信息This antibody is predicting to react with Monkey, Cat, Dog.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Sav1 is a regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. It also plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, Sav1 activates the transcriptional activity of ESR1 through the modulation of its phosphorylation.

数据

SAV1 Antibody (703002) in ICC/IFKnockdown of SAV1 was achieved by transfecting HCT 116 cells with specific siRNA (Silencer® select Product # s34083, s34081). Immunofluorescence analysis was performed on HCT 116 cells (untransfected, panel a-d), transfected with SAV1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-SAV1 Recombinant Rabbit Monoclonal Antibody (Product # 703002, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Significant reduction of signal was observed upon siRNA mediated knockdown (panel i-l) confirming specificity of the antibody to SAV1 (green). The images were captured at 60X magnification.

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