CD49f Recombinant Polyclonal Antibody (5HCLC)

货号：710209

规格：100 µg

价格：4809

产品类型：免疫组化一抗

品牌：Thermo Fisher

物种：人

宿主：兔

抗体亚型：IgG

克隆号：5HCLC

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:500-1:5,000 (WB)；1:100-1:1,000(ICC/IF)

产品详细信息Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息CDf (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signaling. Two transcript variants encoding different isoforms have been found for CDf. Functionally, integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals that affect cellular shape, mobility, and progression through the cell cycle in response to the extracellular matrix. Integrin receptors are composed of alpha and beta subunits, and form structural and functional linkages between the ECM (extra-cellular matrix) and intracellular cytoskeletal linker proteins. Signaling mediated by Intergrin/ECM interactions are also integrated with cellular responses to growth factor signaling to regulate cellular proliferation, cytoskeletal reorganization and other responses necessary for cellular survival. CDf is predominantly expressed by epithelia, and plays a critical structural role in the hemidesmosome. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells.

数据

CD49f Antibody (710209) in ICC/IFImmunofluorescent analysis of Integrin alpha 6 was performed on 90% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0. 25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Integrin alpha 6 Recombinant Rabbit Polyclonal Antibody (Product # 710209) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG secondary antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c is a merged image showing localization at the cell junctions and panel d is a control without primary antibody. The images were captured using a Nikon microscope at 20X magnification.

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参考文献：

1.Molecular reproduction and developmentRFX1 maintains testis cord integrity by regulating the expression of Itga6 in male mouse embryos."710209 was used in immunohistochemistry - paraffin section to determine regulation of expression of Itga6 in male mouse embryos that controls RFX1 maintaining testis cord integrity"2.Molecular pharmaceuticsDevelopment of an in vitro 3D tumor model to study therapeutic efficiency of an anticancer drug.AuthorsShin CS,Kwak B,Han B,Park K

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