Goat anti-Human IgG ，Alexa Fluor 555/山羊抗人IgG，Alexa Fluor 555
货号：GH2305,GH2310
规格：50 μL,100 μL
价格：300,500
产品类型：荧光二抗
品牌：PBM
物种：人
宿主：山羊
抗体亚型：IgG
荧光染料：Alexa Fluor 555
| 抗体类型: | 荧光二抗 | 同型对照： | IgG |
| 浓度 | 2 mg/mL | 用法： | 1-10 µg/mL(ICC/IF);1:2,000(WB); 1-10 µg/mL(IHC) |
产品详细信息
Product will be shipped at Room Temperature.
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
| 数据 |
Human IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21433) Antibody specificity was demonstrated by detection of differential basal expression of IgG across cell lines owing to their inherent genetic constitution. Relative expression of Human IgG was observed in IM-9, ARH-77 and IM-9, ARH-77 conditioned medium (CM) but not in Raji, MOLT-4, Jurkat and Jurkat CM using Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 (Product # A-21433) in Western Blot. IM-9 and ARH-77 express and secrete IgG whereas Raji is known to express IgM. MOLT-4 and Jurkat (T-cell lines) do not express immunoglobulins. (DOI:10.1002/eji.1830100305;10.3791/3573;10.1016/0022-1759(94)00286-6;PMID: 566614) |
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