SAA Human ELISA Kit ELISA试剂盒,SAA Human ELISA Kit/人SAA ELISA试剂盒

2024-10-23

SAA Human ELISA Kit/人SAA ELISA试剂盒

货号:KHA0011,KHA0012,KHA0011C

规格:96 Tests,2X96 Tests,5X96 Tests

价格:7301,10140,24645

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

4 ng/mL

检测范围9.4-600 ng/mL

样本类型/体积

上清液 100 μL

血清 100μL

血浆 100μL

操作时间

1 hr 20 min

测试时间

3 hr

Homogenous (no wash)

No

批间变异系数(CV)7.4%
批内变异系数(CV)6.1%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

5X96 Tests

组份

- 预包被96孔板

- 标准品

- 标准稀释液

- 生物素化检测抗体

- 链霉亲和素-辣根过氧化物酶

- HRP稀释液

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameSAA
Protein aliasesAmyloid fibril protein AA, Amyloid protein A, SAA, serum amyloid A protein, Serum amyloid A-1 protein, Serum amyloid protein A(2-102), Serum amyloid protein A(2-103), Serum amyloid protein A(2-104), Serum amyloid protein A(3-104), Serum amyloid protein A(4-101), tumor protein p53 inducible protein 4

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesPIG4, SAA, SAA1, SAA2, TP53I4
Gene ID(Human) 6288
Gene symbolSAA1
UniProt ID

(Human) P0DJI8

The Human SAA ELISA Kit quantifies human serum amyloid A in serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural 18 kDa isoform and recombinant SAA.人SAA ELISA试剂盒可定量血清,血浆,缓冲液或细胞培养基中的人血清淀粉样蛋白A。 该检测试剂盒识别天然的18 kDa同工型和重组SAA。Principle of the method 方法原理The Human SAA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人SAA固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息The serum amyloid A family comprises a number of differentially expressed apolipoproteins, acute-phase SAA1 and SAA2, the former being the major component in plasma and constitutive SAAs. Although the liver is the primary site of synthesis of both SAA types extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations; SAA rises earlier and more sharply than CRP. Recently, a broader view of SAA expression and function has been emerging.血清淀粉样蛋白A家族包含许多差异表达的载脂蛋白,急性期SAA1和SAA2,前者是血浆和组成型SAA的主要成分。 尽管肝脏是两种SAA类型合成的主要部位,但据报道有肝外生产。 在炎症过程中,体内浓度增加了多达1000倍。 多项研究表明了其在各种疾病的诊断和监测中的重要性。 通常会结合正常CRP浓度检测到病理性SAA值。 SAA的上升比CRP上升得更快,也更迅速。 最近,已经出现了对SAA表达和功能的更广泛的了解。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

SAA Human ELISA KitRepresentative standard curve for Human SAA ELISA

技术参数
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