Cytokeratin 14 Monoclonal Antibody (SP53)/KRT14单抗

货号：MA5-16370

规格：500 µL

价格：7381

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to C-terminus of h

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

克隆号：SP53

荧光染料：其它

类型:	单抗	同型对照：	IgG
浓度：		用法：	1:100-1:1900(Flow);1:2000(ICC);1:2000(IF);1:2000(IHC(F));1:100(IHC(P));1:25(WB)

产品详细信息Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skin, squamous cell carcinoma or prostate tissue.靶标信息Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. skin) and non-keratinizing cells (ie. mesothelial cells). At least 20 different CKs can be identified. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). Belonging to the type I subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.

数据

Cytokeratin 14 Antibody (MA5-16370) in WBWestern blot analysis of A431 Cells using anti-Keratin 14 Monoclonal Antibody (Product # MA5-16370). The recommened dilution for this antibody in western blot applications is 1:25. Cytokeratin 14 Antibody (MA5-16370) in IHCImmunohistochemical analysis of Keratin 14 using anti-Keratin 14 Monoclonal Antibody (Product # MA5-16370) in Prostate Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.

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