KRT14 Polyclonal Antibody/KRT14多抗

货号：PA5-99310

规格：100 µL

价格：4711

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：A synthesized peptide derived from human Keratin 1

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：	IgG
浓度：	1 mg/mL	用法：	1:100-1:500(ICC);1:100-1:500(IF);1:50-1:200(IHC(P));1:500-1:2000(WB)

靶标信息Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. skin) and non-keratinizing cells (ie. mesothelial cells). At least 20 different CKs can be identified. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). Belonging to the type I subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

KRT14 Antibody (PA5-99310) in WBWestern blot analysis of KRT14 in NIH-3T3 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with KRT14 polyclonal antibody (Product # PA5-99310). KRT14 Antibody (PA5-99310) in IFImmunofluorescent analysis of KRT14 in sw480. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with KRT14 polyclonal antibody (Product # PA5-99310) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600. KRT14 Antibody (PA5-99310) in IHC (P)Immunohistochemistry analysis of paraffin-embedded KRT14 in human colon cancer tissues sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with KRT14 polyclonal antibody (Product # PA5-99310) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

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