IL-12 p70 Rat ELISA Kit ELISA试剂盒,IL-12 p70 Rat ELISA Kit/大鼠IL-12 p70 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-12 p70 Rat ELISA Kit ELISA试剂盒,IL-12 p70 Rat ELISA Kit/大鼠IL-12 p70 ELISA试剂盒

2024-10-23

IL-12 p70 Rat ELISA Kit/大鼠IL-12 p70 ELISA试剂盒

货号：KRC0121,KRC0122

规格：96 Tests,2X96 Tests

价格：7100,11232

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：大鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<3 pg/mL
检测范围	15.6-1000 pg/mL
样本类型/体积	上清液 100 μL 血清 10 μL 血浆 10 μL
操作时间	1 hr 20 min
测试时间	4 hr
Homogenous (no wash)	No
批间变异系数（CV）	5.1%
批内变异系数（CV）	4.2%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	2X96 Tests
组份	- 预包被96孔板 - 标准品 - 标准稀释液 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 辣根过氧化物酶稀释剂 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-12 p70
Protein aliases	CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, interleukin 12 p35 subunit, interleukin-12 p40, Interleukin-12 subunit alpha, interleukin-12 subunit beta
物种（Tested）	大鼠
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	Il12, Il12a
Gene ID	(Rat) 84405,(Rat) 64546
Gene symbol	Il12a, Il12b
UniProt ID	(Rat) Q9R103

The Rat Interleukin 12+p40 (Rt IL-12+p40) ELISA quantitates Rt IL-12+p40 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt IL-12+p40.大鼠白介素12 + p40（大鼠IL-12 + p40）ELISA定量了人血清，血浆，缓冲液或细胞培养基中的大鼠IL-12 + p40。该检测试剂盒将专门识别天然和重组大鼠IL-12 + p40。Principle of the method 方法原理The Rat IL-12+p40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.大鼠IL-12 + p40固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. IL-12 p70 is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Human and mouse IL-12 p40 share about 70% amino acid sequence homology.白介素12（IL-12）是一种异源二聚体70 kDa细胞因子，由两条共价连接的糖基化链组成，分子量分别为40kD（p40）和35-kD（p35）。 IL-12主要由单核细胞，巨噬细胞和树突状细胞响应细菌产品（如脂多糖（LPS）），细胞内病原体或与活化T细胞相互作用而产生。最初发现IL-12是因为它具有诱导干扰素-γ（IFN-g）产生，细胞增殖以及由自然杀伤细胞和T细胞介导的细胞毒性的能力。研究表明，IL-12在Th1反应的发展中也起着关键作用，导致IFN-g和IL-2的产生。这些细胞因子可进而促进T细胞反应和巨噬细胞活化。使用双启动子表达系统，在杆状病毒感染的昆虫细胞中产生重组小鼠IL-12 p70，作为前体p35和p40亚基的真正异源二聚体。 IL-12 p70与其他可用形式的蛋白质不同，因为它表达为真正的异源二聚体，而不是亚基通过人工接头连接的单链伪异源二聚体。淋巴细胞对此细胞因子的反应是由转录蛋白STAT4的激活剂介导的。发现一氧化氮合酶2A（NOS2A / NOS2）是先天免疫中该细胞因子信号传导过程所必需的。人和小鼠IL-12 p40共有约70％的氨基酸序列同源性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-12 p70 Rat ELISA KitRepresentative standard curve for Rat IL-12 p40 ELISA

技术参数

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IL-12 p70 Rat ELISA Kit ELISA试剂盒,IL-12 p70 Rat ELISA Kit/大鼠IL-12 p70 ELISA试剂盒

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