Resistin Human ELISA Kit/人抵抗素ELISA试剂盒
货号：KHP0051
规格：96 tests
价格：8779
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 100 pg/mL |
| 检测范围 | 0.125-8 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 25 μL 血浆 25 μL |
操作时间 | 1 hr 20 min |
测试时间 | 3 hr 10 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | 5.6% |
| 批内变异系数（CV） | 3.7% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 标准稀释液 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 辣根过氧化物酶稀释剂 - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Resistin |
| Protein aliases | Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, found in inflammatory zone 3, Resistin, resistin delta2 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | ADSF, FIZZ3, HXCP1, RETN, RETN1, RSTN, UNQ407/PRO1199, XCP1 |
| Gene ID | (Human) 56729 |
| Gene symbol | RETN |
| UniProt ID | (Human) Q9HD89 |
The Human Resistin ELISA quantitates Hu Resistin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Resistin.人抵抗素ELISA可以定量人血清，血浆，缓冲液或细胞培养基中的人抵抗素。 该检测试剂盒将专门识别天然和重组的人抵抗素。Principle of the method 方法原理The Human Resistin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人抵抗素固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Adipose tissue is an energy reserve in animals and is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice. Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.脂肪组织是动物的能量储备，并且受到严格监管。脂肪细胞产生并分泌许多生理上重要的蛋白质，例如脂蛋白酶（LPL），瘦素，30 kDa的脂肪细胞补体相关蛋白（Acrp30），周脂蛋白和抵抗素。抵抗素，也称为脂肪细胞分泌因子（ADSF）和FIZZ3，是由脂肪细胞特异性分泌的细胞因子。虽然抵抗素的功能尚不十分清楚，但它似乎具有关键的旁分泌作用，并且在2型糖尿病中具有作用。研究表明，在肥胖的糖尿病小鼠中，血清抵抗素水平升高，这对胰岛素具有拮抗作用。其他研究表明，抵抗素会引起小鼠胰岛素抵抗和葡萄糖不耐症。抵抗素基因表达需要C / EBP alpha，这对于表达是必需的和充分的。研究表明，已显示具有组蛋白乙酰基转移酶（HAT）活性的共激活剂CBP和p300在抵抗素启动子处将组蛋白充分乙酰化，表明它们在激活染色质以抵抗抵抗素转录方面发挥了作用。已显示许多PPARγ和RXR配体下调脂肪细胞中抵抗素的表达。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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