RBP4 Human ELISA Kit ELISA试剂盒,RBP4 Human ELISA Kit/人RBP4 ELISA试剂盒

2024-10-23

RBP4 Human ELISA Kit/人RBP4 ELISA试剂盒

货号:BMS2199

规格:96 tests

价格:6120

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.034 ng/mL

检测范围0.063-4 ng/mL

样本类型/体积

血清 10 μL

血浆 10 μL

上清液 10 μL

细胞裂解液 10 μL

操作时间

1 hr 20 min

测试时间

3 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)8.1%
批内变异系数(CV)5%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 对照

- 胶板盖

储存温度

2-8℃

Protein nameRBP4
Protein aliasesPlasma retinol-binding protein, Plasma retinol-binding protein(1-176), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-182), PRBP, RBP, retinol binding protein 4, plasma, Retinol-binding protein 4, retinol-binding protein 4, interstitial

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesMCOPCB10, PRO2222, RBP4, RDCCAS
Gene ID(Human) 5950
Gene symbolRBP4
UniProt ID(Human) P02753
The Human Retinol-binding protein 4 (HuRBP-4) ELISA quantitates Hu RBP-4 in human serum, plasma, cell lysates, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu RBP-4.人视黄醇结合蛋白4(人RBP-4)ELISA定量了人血清,血浆,细胞裂解液或细胞培养基中的人RBP-4。 该检测试剂盒将专门识别天然和重组人RBP-4。Principle of the method 方法原理The Human RBP-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人RBP-4固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息This protein belongs to the lipocalin family and is the specific carrier for retinol in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein post translationally and results in defective delivery and supply to the epidermal cells.该蛋白属于脂环蛋白家族,是血液中视黄醇的特定载体。 它将视黄醇从肝脏储存处输送到周围组织。 在血浆中,RBP-视黄醇复合物与运甲状腺素蛋白相互作用,可通过肾小球滤过来防止其丢失。 维生素A的缺乏会阻碍翻译后结合蛋白的分泌,并导致有缺陷的递送和供应给表皮细胞。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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