Granzyme A Human ELISA Kit/人Granzyme A ELISA试剂盒

货号：BMS2232

规格：96 Tests

价格：6150

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.73 pg/mL
检测范围	7.8-500 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	8.5%
批内变异系数（CV）	4.9%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 tests
组份	- 预包被96孔板 - 标准品（2瓶） - 标准稀释液 - 生物素化检测抗体 - Streptavidin-HRP - HRP稀释液 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	Granzyme A
Protein aliases	CTL tryptase, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, Hanukkah factor, H factor, HF, Cytotoxic T-lymphocyte-associated serine esterase-3, granzyme 1, Granzyme A, Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3, Hanukah factor serine protease), granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CTLA3, GZMA, HFSP
Gene ID	(Human) 3001
Gene symbol	GZMA
UniProt ID	(Human) P12544

The Human Granzyme A ELISA quantitates Hu Granzyme A in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Granzyme A.人粒酶A ELISA可以定量人血清，血浆或细胞培养基中的人粒酶A。 该检测试剂盒将专门识别天然和重组人粒酶A。Principle of the method 方法原理The Human Granzyme A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人粒酶A固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，该第二抗体与与捕获抗体不同的表位上的靶标结合。 已将结合酶掺入测定中。 经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.细胞溶解性T淋巴细胞（CTL）和自然杀伤（NK）细胞具有识别，结合和裂解特定靶细胞的卓越能力。 人们认为它们通过裂解表面“非自身”抗原上的细胞来保护其宿主，这些抗原通常是细胞内病原体感染导致的肽或蛋白质。 此处描述的蛋白质是T细胞和天然杀伤细胞特异性的丝氨酸蛋白酶，可充当细胞毒性T淋巴细胞和天然杀伤细胞裂解靶细胞所需的常见成分。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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