CD40 (Soluble) Human ELISA Kit ELISA试剂盒,CD40 (Soluble) Human ELISA Kit/人CD40(可溶)ELISA试剂盒

2024-10-23

CD40 (Soluble) Human ELISA Kit/人CD40(可溶)ELISA试剂盒

货号:BMS265,BMS265TEN

规格:96 Tests,10X96 Tests

价格:6120,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

1.3 pg/mL

检测范围7.8-500 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

羊水 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

3 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)7%
批内变异系数(CV)5.5%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameCD40
Protein aliasesB cell surface antigen CD40, B cell-associated molecule, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CD40, CD40 molecule, TNF receptor superfamily member 5, Tumor necrosis factor receptor superfamily member 5

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesBp50, CD40, CDW40, p50, TNFRSF5
Gene ID(Human) 958
Gene symbolCD40
UniProt ID(Human) P25942
The Human CD40 ELISA quantitates Hu CD40 in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD40.人CD40ELISA可以定量血清,血浆,羊水或细胞培养基中的CD40。 该检测试剂盒将专门识别天然和重组人CD40。Principle of the method 方法原理The Human CD40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人CD40固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.CD40抗原是单链糖蛋白,是肿瘤坏死因子/神经生长因子超家族的成员,与霍奇金氏病相关抗原CD30显示出显著的同源性。 CD40存在于除浆细胞以外的所有B细胞中,并且还存在于某些上皮细胞,癌和淋巴样树突细胞中。已发现CD40在介导多种免疫和炎症反应中至关重要,包括T细胞依赖性免疫球蛋白类别转换,记忆B细胞发育和生发中心形成。据报道,AT钩转录因子AKNA协调调节CD40的表达,这可能对同型细胞相互作用很重要。衔接蛋白TNFR2与CD40相互作用,并充当信号转导的介质。发现CD40及其配体的相互作用对于淀粉样β诱导的小胶质细胞活化是必需的,因此被认为是阿尔茨海默氏病发病机理中的早期事件。已经报道了编码不同同工型的CD40的两个选择性剪接的转录变体。与CD40功能障碍相关的疾病包括3型高Igm免疫缺陷和CD40配体缺陷。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

CD40 (Soluble) Human ELISA KitRepresentative standard curve for Human CD40 ELISA

技术参数
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