APRIL Human ELISA Kit ELISA试剂盒,APRIL Human ELISA Kit/人APRIL ELISA试剂盒

2024-10-23

APRIL Human ELISA Kit/人APRIL ELISA试剂盒

货号: BMS2008,BMS2008TEN

规格:96 Tests,10X96 Tests

价格:6120,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.4 ng/mL

检测范围0.78-50.0 ng/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

3 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)7.1%
批内变异系数(CV)8.1%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 裂解液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameAPRIL
Protein aliasesA proliferation-inducing ligand, APRIL, CD256, TALL-2, TNF- and APOL-related leukocyte expressed ligand 2, TNF-related death ligand 1, TRDL-1, tumor necrosis factor (ligand) superfamily, member 13, tumor necrosis factor ligand 7B, Tumor necrosis factor ligand superfamily member 13, tumor necrosis factor-like protein ZTNF2, tumor necrosis factor-related death ligand-1

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesAPRIL, CD256, TALL-2, TALL2, TNFSF13, TNLG7B, TRDL-1, UNQ383/PRO715, ZTNF2
Gene ID(Human) 8741
Gene symbolTNFSF13
UniProt ID(Human) O75888
The Human APRIL (Hu A PRoliferation-Inducing Ligand)) ELISA quantitates Hu APRIL in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu APRIL.人APRIL(人APRIL)ELISA定量测定人血清,血浆,缓冲溶液或细胞培养基中的人APRIL。 该检测试剂盒将专门识别天然和重组人APRIL。Principle of the method 方法原理The Human APRIL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人APRIL固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for A PRoliferation-Inducing Ligand), TALL2 (for TNF and ApoL-related Leukocyte-expressed Ligand 2), and TRDL 1a (for TNF related death ligand 1a) in human and mouse. Two receptors for APRIL were recently identified and designated TACI and BCMA. APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kB, and induces cell death. APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer.TNF超家族成员调节免疫反应并诱导细胞凋亡。 最近,几个小组确定了TNF家族的一个新成员,并将其命名为APRIL(用于诱导增殖的配体),TALL2(用于TNF和与ApoL相关的白细胞表达的配体2)和TRDL 1a(用于TNF相关的死亡配体1a)。 在人和小鼠中,最近发现了两种APRIL受体,分别命名为TACI和BCMA。 APRIL刺激B和T细胞增殖,触发体液免疫反应,激活NF-kB,并诱导细胞死亡。 APRIL及其BlyS及其受体BCMA和TACI的近亲与自身免疫性疾病和癌症有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

APRIL Human ELISA KitRepresentative standard curve for Human APRIL ELISA

技术参数
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