Caspase 8/FLICE Human ELISA Kit ELISA试剂盒,Caspase 8/FLICE Human ELISA Kit/人半胱天冬酶8 / FLICE ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Caspase 8/FLICE Human ELISA Kit ELISA试剂盒,Caspase 8/FLICE Human ELISA Kit/人半胱天冬酶8 / FLICE ELISA试剂盒

2024-10-23

Caspase 8/FLICE Human ELISA Kit/人半胱天冬酶8 / FLICE ELISA试剂盒

货号：BMS2024,BMS2024TEN

规格：96 Tests,10X96 Tests

价格：6120,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.10 ng/mL
检测范围	0.16-10.0 ng/mL
样本类型/体积	血清 50 μL 上清液 50 μL 细胞裂解液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 15 min
Homogenous (no wash)	No
批间变异系数（CV）	8.5%
批内变异系数（CV）	6.7%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 检测抗体 - 抗-兔HRP共轭物 - 裂解液 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	Caspase 8
Protein aliases	Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, caspase 8, apoptosis-related cysteine peptidase, apoptosis-related cysteine protease, Caspase-8, Caspase-8 subunit p10, Caspase-8 subunit p18, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MACH, MACH-alpha-1/2/3 protein, MACH-beta-1/2/3/4 protein, MORT1-associated ced-3 homolog
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	ALPS2B, CAP4, Casp-8, CASP8, FLICE, MACH, MCH5
Gene ID	(Human) 841
Gene symbol	CASP8
UniProt ID	(Human) Q14790

The Human Caspase 8/FLICE ELISA quantitates Hu Caspase 8 in human serum, cell lysate or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Caspase 8.人半胱天冬酶8 / FLICE ELISA可以定量人血清，细胞裂解液或细胞培养基中的人半胱天冬酶8。该检测试剂盒将专门识别天然和重组人半光天冬酶8。Principle of the method 方法原理The Human Caspase 8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人半光天冬酶8固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Many alternatively spliced transcript variants encoding different isoforms have been described for Caspase 8, however, not all variants have had their full-length sequences determined.Caspase 8（CASP8）是半胱氨酸天冬氨酸蛋白酶（caspase）家族的成员。半胱天冬酶的顺序激活在细胞凋亡的执行阶段起着核心作用。胱天蛋白酶以由前结构域，大的蛋白酶亚基和小的蛋白酶亚基组成的非活性酶的形式存在。半胱天冬酶的激活需要在保守的内部天冬氨酸残基上进行蛋白水解处理，以生成由大亚基和小亚基组成的异二聚酶。 Caspase 8参与由Fas和各种凋亡刺激物诱导的程序性细胞死亡。 Caspase 8的N末端FADD样死亡效应域表明它可能与Fas相互作用蛋白FADD相互作用。在来自亨廷顿病患者的受影响脑区域的不溶部分中检测到胱天蛋白酶8，但在正常对照者中未检测到，这暗示了神经变性疾病中的作用。 Caspase 8通过以Caspase 8蛋白原形式串联存在的类似DED域与FADD的死亡效应域（DED）结合。然后，活化的半胱天冬酶8激活包括Caspase 9在内的其他下游半胱天冬酶，从而使细胞发生凋亡。另外，Caspase 8还与Jurkat细胞和扁桃体反应。 Caspase 8的过表达诱导凋亡，该凋亡可被ICE家族特异的抑制剂阻断。已经针对半胱天冬酶8描述了许多编码不同同工型的可变剪接的转录变体，但是，并不是所有的变体都已确定了其全长序列。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

Caspase 8/FLICE Human ELISA KitRepresentative standard curve for Human Caspase-8 ELISA

技术参数

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Caspase 8/FLICE Human ELISA Kit ELISA试剂盒,Caspase 8/FLICE Human ELISA Kit/人半胱天冬酶8 / FLICE ELISA试剂盒

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