IL-15 Human ELISA Kit ELISA试剂盒,IL-15 Human ELISA Kit/人IL-15 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-15 Human ELISA Kit ELISA试剂盒,IL-15 Human ELISA Kit/人IL-15 ELISA试剂盒

2024-10-23

IL-15 Human ELISA Kit/人IL-15 ELISA试剂盒

货号：BMS2106,BMS2106TEN

规格：96 Tests,10X96 Tests

价格：6120,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	3.4 pg/mL
检测范围	15.6-1,000.0 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	9.7%
批内变异系数（CV）	9.3%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-15
Protein aliases	IL-15, Interleukin-15
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	IL-15, IL15
Gene ID	(Human) 3600
Gene symbol	IL15
UniProt ID	(Human) P40933

The Human Interleukin-15 (IL-15) ELISA quantitates Hu IL-15 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-15.人白介素15（IL-15）ELISA定量测定人血清，血浆，缓冲液或细胞培养基中的人IL-15。该检测试剂盒将只识别天然和重组人IL-15。Principle of the method 方法原理The Human IL-15 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-15固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.IL-15（白介素15）是一种调节T和自然杀伤细胞活化和增殖的细胞因子。 IL-15和IL-2具有许多生物学活性，因为它们都结合了常见的造血素受体亚基，并且可能竞争同一个受体，从而对彼此的活性产生负面调节。 CD8 +存储单元的数量显示受IL-15和IL-2之间的平衡控制。 IL-15诱导JAK激酶的激活，以及转录激活因子STAT3，STAT5和STAT6的磷酸化和激活。在小鼠中，研究表明IL-15可能通过STAT6的转录激活活性来增加凋亡抑制剂Bcl-xL的表达，从而阻止凋亡。 IL-15在T和B淋巴细胞，自然杀伤细胞，巨噬细胞和单核细胞的生长和分化以及许多重要的细胞内信号分子的激活中起着重要作用。因此，IL-15对于免疫反应，同种异体移植排斥和自身免疫性疾病的发病机制可能是必不可少的。此外，IL-15是广泛表达的促炎性细胞因子，并且已显示出它在几种炎性疾病中起作用，包括类风湿性关节炎，牛皮癣和肺炎性疾病。新兴数据表明，IL-15可以作为良好的治疗靶标，因为在牛皮癣和糖尿病模型中，IL-15中和作用似乎是有益的。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-15 Human ELISA KitRepresentative standard curve for Human IL-15 ELISA

技术参数

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IL-15 Human ELISA Kit ELISA试剂盒,IL-15 Human ELISA Kit/人IL-15 ELISA试剂盒

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