Zeba™ Micro Spin Desalting Columns, 7K MWCO, 75 µL,Zeba™ Micro Spin Desalting Columns, 7K MWCO, 75 µL/脱盐离心柱-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Zeba™ Micro Spin Desalting Columns, 7K MWCO, 75 µL,Zeba™ Micro Spin Desalting Columns, 7K MWCO, 75 µL/脱盐离心柱

2024-10-23

Zeba™ Micro Spin Desalting Columns, 7K MWCO, 75 µL/脱盐离心柱

货号：89877,89878

规格：25 columns,50 columns

价格：3974,7067

产品类型：蛋白提取/纯化

品牌：Thermo Fisher

Thermo Scientific的Zeba脱盐离心柱（7K MWCO，5mL）采用聚丙烯材料制成，内含获得专利的高性能体积排阻色谱层析树脂填料，基于离心工作原理工作，能够出色地完成蛋白脱盐和回收。Zeba脱盐离心柱（7K MWCO，5mL）的特性：•高回收率—低结合树脂实现最大蛋白回收率•快速—无需进行重力流实验中所需的组分筛选或等待蛋白出现•简单易用—无需繁琐的离心柱制备或平衡过程•灵活—离心柱、离心板和色谱层析柱多种形式，满足种类广泛的不同需求•价格经济—成本低于其他市售色谱层析柱，性能更高即使是经过高倍稀释(25 µg/mL)的蛋白样品，Zeba脱盐离心柱（7K MWCO，0.5mL）也能实现95%以上盐类与其他小分子(< 1000 MW)的滞留（清除）效果，以及蛋白及其他大分子(> 7000 MW)的良好回收效果。可选形式：•离心柱—75 µL、0.5mL (微量离心柱)、2mL、5mL、10mL离心柱•色谱层析柱—兼容自动化液相层析系统，通过注射器进行上样 (了解色谱层析柱更多信息)•96孔离心板—预装型96孔过滤平板，兼容手动或自动纯化操作。该平板能够在孔与孔、板与板之间快速可靠地实现小规模、高通量分离操作。(参见Zeba 96孔离心板数据)

数据：

Protein recovery and desalting efficiencyProtein samples (1mg/mL) were prepared in 1.0M NaCl and 100µL samples were desalted using Zeba Spin Desalting Plates, 7K MWCO. Results were analyzed by BCA Protein Assay and conductivity measurements.

Better performance with Zeba Spin Desalting ColumnsThermo Scientific Zeba Columns provide higher protein recovery and less sample dilution over a wider range of sample concentrations and volume compared to alternative products. Zeba Spin Desalting Columns (7K MWCO, 10mL) and Disposable PD-10 Desalting Columns (#17-0851-01, GE Healthcare) were used to desalt 1.5, 2.5 and 3.5mL samples of bovine serum albumin (BSA) at concentrations of 0.04, 0.2, and 1mg/mL. Desalting was performed according to each manufacturer’s recommended protocols for either spin (centrifuge) or drip (gravity) procedures. Three sets of columns were equilibrated in final buffer and then loaded with 1.5, 2.5, and 3.5mL samples. For each electrophoresis gel, an aliquot of starting sample equal to 1µg of BSA was loaded in Lane 1 as the Load Control; all other desalted samples were loaded in the gel at the same volume as the Load Control. Differences in intensity between lanes are a combination of protein recovery and sample dilution caused by desalting.

技术参数

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Zeba™ Micro Spin Desalting Columns, 7K MWCO, 75 µL,Zeba™ Micro Spin Desalting Columns, 7K MWCO, 75 µL/脱盐离心柱

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