IL-5 Human ELISA Kit ELISA试剂盒,IL-5 Human ELISA Kit/人IL-5 ELISA试剂盒

2024-10-23

IL-5 Human ELISA Kit/人IL-5 ELISA试剂盒

货号:BMS278,BMS278TEN

规格:96 Tests,10X96 Tests

价格:6120,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

1.5 pg/mL

检测范围7.8-500 pg/mL

样本类型/体积

血清 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

3 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)6.8%
批内变异系数(CV)6.6%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameIL-5
Protein aliasesB-cell differentiation factor I, colony-stimulating factor, eosinophil, Eosinophil differentiation factor, IL-5, Interleukin-5, T-cell replacing factor, TRF

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesEDF, IL-5, IL5, TRF
Gene ID(Human) 3567
Gene symbolIL5
UniProt ID(Human) P05113
The Human Interleukin-5 (Hu IL-5) ELISA quantitates Hu IL-5 in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-5.人白介素5(人IL-5)ELISA定量了人血清或细胞培养基中的人IL-5。 该检测试剂盒将专门识别天然和重组的人IL-5。Principle of the method 方法原理The Human IL-5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-5固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.IL-5是一种细胞因子,可同时充当B细胞和嗜酸性粒细胞的生长和分化因子。 IL-5细胞因子是嗜曙红细胞生成,嗜酸性粒细胞成熟和激活的主要调节因子。 据报道,这种细胞因子的产生增加与哮喘或嗜酸性粒细胞增多综合征有关。 IL-5细胞因子的受体是异二聚体,其β亚基与白细胞介素3(IL3)和集落刺激因子2(CSF2 / GM-CSF)的受体共有。 IL-5与白介素4(IL4),白介素13(IL13)和CSF2一起,在5号染色体上形成细胞因子基因簇。发现IL-5,IL4和IL13受长距离协同调节。 调控元件在5q31号染色体上分布了120千个碱基。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

IL-5 Human ELISA KitRepresentative standard curve for Human IL-5 ELISA

技术参数
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