CD31/PECAM (Soluble) Human ELISA Kit ELISA试剂盒,CD31/PECAM (Soluble) Human ELISA Kit/人CD31/PECAM (可溶) ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

CD31/PECAM (Soluble) Human ELISA Kit ELISA试剂盒,CD31/PECAM (Soluble) Human ELISA Kit/人CD31/PECAM (可溶) ELISA试剂盒

2024-10-23

CD31/PECAM (Soluble) Human ELISA Kit/人CD31/PECAM (可溶) ELISA试剂盒

货号：BMS229,BMS229TEN

规格：96 Tests,10X96 Tests

价格：6120,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.06 ng/mL
检测范围	0.47-30.0 ng/mL
样本类型/体积	血清 10 μL 血浆 10 μL 尿液 10 μL 上清液 10 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	7.4%
批内变异系数（CV）	1.7%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 对照 - 胶板盖
储存温度	2-8℃
Protein name	CD31 (PECAM-1)
Protein aliases	CD31, CD31 antigen, EndoCAM, GPIIA', PECA1, PECAM-1, Platelet endothelial cell adhesion molecule, platelet/endothelial cell adhesion molecule 1
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	CD31, CD31/EndoCAM, endoCAM, GPIIA', PECA1, PECAM-1, PECAM1
Gene ID	(Human) 5175
Gene symbol	PECAM1
UniProt ID	(Human) P16284

The Human Soluble CD31/Platelet Endothelial Cell Adhesion Molecule(Hu sCD31/PECAM) ELISA quantitates Hu sCD31/PECAM in human serum, plasma, cell culture supernatants, urine, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sCD31/PECAM.人可溶性CD31 /血小板内皮细胞粘附分子（人sCD31 / PECAM）ELISA定量分析了人血清，血浆，细胞培养上清液，尿液或其他体液中的人sCD31 / PECAM。该检测试剂盒将专门识别天然和重组人sCD31 / PECAM。Principle of the method 方法原理The Human sCD31/PECAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人sCD31 / PECAM固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。通过将第二（检测）抗体与与捕获抗体不同的表位上的靶标结合形成三明治。与酶缀合的抗体结合形成的三明治。经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.CD31（血小板内皮细胞粘附分子-1，PECAM-1）是一种抑制性共受体，通过基于双重免疫受体酪氨酸的抑制性基序（ITIM）参与T细胞和B细胞信号的调节，在相关激酶介导的磷酸化作用下提供了停靠位点用于蛋白质酪氨酸磷酸酶。 CD31在血管腔内无处不在表达，并且主要位于相邻细胞之间的交界处。 CD31的N端Ig样结构域负责其同质结合，这在细胞间相互作用中起着重要作用。 CD31是一种多功能分子，在整合素介导的细胞粘附，跨内皮迁移，血管生成，凋亡，免疫受体信号的负调节，自身免疫，巨噬细胞吞噬作用，IgE介导的过敏反应和血栓形成中具有多种作用。它是血管系统中的关键调节分子之一。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

CD31/PECAM (Soluble) Human ELISA KitRepresentative standard curve for Human soluble PECAM-1 ELISA

技术参数

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CD31/PECAM (Soluble) Human ELISA Kit ELISA试剂盒,CD31/PECAM (Soluble) Human ELISA Kit/人CD31/PECAM (可溶) ELISA试剂盒

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