IL-33 Mouse ELISA Kit ELISA试剂盒,IL-33 Mouse ELISA Kit/小鼠IL-33 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-33 Mouse ELISA Kit ELISA试剂盒,IL-33 Mouse ELISA Kit/小鼠IL-33 ELISA试剂盒

2024-10-23

IL-33 Mouse ELISA Kit/小鼠IL-33 ELISA试剂盒

货号：BMS6025,BMS6025TEN

规格：96 Tests,10X96 Tests

价格：6120,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	11.2 pg/mL
检测范围	46.9-3,000 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	3.6%
批内变异系数（CV）	6.7%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-33
Protein aliases	IL-33, Interleukin-33, Interleukin-33(102-266), Interleukin-33(109-266), nuclear factor from high endothelial venules
物种（Tested）	小鼠
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	9230117N10Rik, Il-33, Il1f11, Il33, NF-HEV
Gene ID	(Mouse) 77125
Gene symbol	Il33
UniProt ID	(Mouse) Q8BVZ5

The Mouse Interleukin-33 (Ms IL-33) ELISA quantitates Ms IL-33 in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-33.小鼠白介素33（小鼠IL-33）ELISA定量检测小鼠血清，血浆，缓冲液或细胞培养基中的小鼠IL-33。该检测试剂盒将专门识别天然和重组小鼠IL-33。Principle of the method 方法原理The Mouse IL-33 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠IL-33固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.IL-33（白介素-33）是一种270个氨基酸的高分化蛋白，属于具有IL-1样C末端结构域的IL-1家族。 IL-33是一种双重功能蛋白，可以同时充当促炎细胞因子和具有转录调控特性的细胞内核因子。 IL-33与IL1RL1 / ST2结合并通过IL1RL1 / ST2发出信号，其刺激募集MYD88，IRAK1，IRAK4和TRAF6。 IL-33激活NF-κB和MAP激酶，并驱动体外极化的TH2细胞产生TH2相关的细胞因子。在体内，IL-33诱导IL-4，IL-5和IL-13的表达，并导致粘膜器官发生严重的病理变化。 IL-33被CASP1蛋白水解转化为成熟形式，并在扁桃体，派伊尔氏淋巴结和肠系膜淋巴结中发现的高内皮微静脉中高度表达，并且在胎盘中几乎无法检测到。长期对小鼠进行IL-33治疗会导致嗜酸性粒细胞增多，脾肿大和粘膜器官（如肺，食道和小肠）的严重病理变化。最近的实验表明，IL-33还可以与异染色质共定位，并具有转录抑制活性，这表明IL-33既可以作为促炎细胞因子，又可以作为具有转录调控特性的细胞内核因子。尽管具有预期的分子量，但IL-33在SDS-PAGE中通常会以较高的分子量运行。研究表明，IL-33还可以与异染色质共定位，并具有转录抑制因子活性，表明IL-33既可以充当促炎细胞因子，又可以具有转录调控特性的细胞内核因子。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-33 Mouse ELISA KitRepresentative standard curve for Mouse IL-33 ELISA

技术参数

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IL-33 Mouse ELISA Kit ELISA试剂盒,IL-33 Mouse ELISA Kit/小鼠IL-33 ELISA试剂盒

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