Arginase-1 Human ELISA Kit ELISA试剂盒,Arginase-1 Human ELISA Kit/人Arginase-1 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Arginase-1 Human ELISA Kit ELISA试剂盒,Arginase-1 Human ELISA Kit/人Arginase-1 ELISA试剂盒

2024-10-23

Arginase-1 Human ELISA Kit/人Arginase-1 ELISA试剂盒

货号：BMS2216,BMS2216TEN

规格：96 Tests,10X96 Tests

价格：5970,36168

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	20.81 pg/mL
检测范围	78.1–5000 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 细胞裂解液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	8.5%
批内变异系数（CV）	6.6%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品（2瓶） - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - Streptavidin-HRP - 洗涤液 - 底物溶液 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	Arginase 1
Protein aliases	arginase, liver, liver-type arginase, type I arginase, Arginase-1, Liver-type arginase, Type I arginase
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	ARG1
Gene ID	(Human) 383
Gene symbol	ARG1
UniProt ID	(Human) P05089

The Arginase-1 Human ELISA Kit quantitates Hu Renin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Arginase-1.Arginase-1人ELISA试剂盒可定量人血清，血浆或细胞培养基中的人Renin。该检测试剂盒将专门识别天然和重组人Arginase-1。Principle of the method 方法原理The Human Arginase-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人Arginase-1固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，该第二抗体与与捕获抗体不同的表位上的靶标结合。已将结合酶掺入测定中。经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.Arginase-1（Arg1）是一种35 kDa的酶，可将L-精氨酸转化为尿素和L-鸟氨酸，这是尿素循环的最后一步。所得的多胺对于细胞增殖和去除蛋白质降解产生的毒素非常重要。通过降解精氨酸，精氨酸酶1剥夺了NO合酶的底物，并下调了一氧化氮的产生。在人和小鼠中，精氨酸酶1均在肝脏，嗜中性粒细胞，髓样来源的抑制细胞（MDSC）和神经干细胞中表达。在人类中，据报道在血液中性粒细胞中表达，但在CCR3 +粒细胞中不表达。在小鼠中，精氨酸酶1的表达是交替激活的巨噬细胞（M2a）的标志之一。精氨酸酶-1可以在浸润肿瘤的髓样细胞中表达，并且通常在大多数肝细胞癌中发现。精氨酸酶1的缺陷是精氨酸血症的原因，精氨酸血症是一种以高氨血症为特征的常染色体隐性遗传疾病。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-45017.htm

特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系删除，本平台不提供信息存储服务。

Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.

产品询价需求提交

Arginase-1 Human ELISA Kit ELISA试剂盒,Arginase-1 Human ELISA Kit/人Arginase-1 ELISA试剂盒

相关推荐