IL-12p70 Human ProQuantum Immunoassay Kit,IL-12p70 Human ProQuantum Immunoassay Kit/人IL-12p70 ProQuantum免疫测定试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-12p70 Human ProQuantum Immunoassay Kit,IL-12p70 Human ProQuantum Immunoassay Kit/人IL-12p70 ProQuantum免疫测定试剂盒

2024-10-23

IL-12p70 Human ProQuantum Immunoassay Kit/人IL-12p70 ProQuantum免疫测定试剂盒

货号：A35591,A355915

规格：96 Tests,5X96 Tests

价格：6561,29500

产品类型：Proquantum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<0.01 pg/mL
检测范围	0.0128 - 5000 pg/mL
样本类型/体积	血浆 2-5 μL 血清 2-5 μL 上清液 2-5 μL
操作时间	30 min
测试时间	2 hr
Homogenous (no wash)	Yes
仪器设备	qPCR Instruments
规格	5 X 96 Tests
组份	- 分析稀释缓冲液 - 抗体结合物 A - 抗体结合物 B - 抗体结合稀释缓冲液 - 标准品 - 预混料 - 连接酶
储存温度	-20℃
运输条件	干冰
Protein name	IL-12 p70
Protein aliases	CLMF p35, CLMF p40, cytotoxic lymphocyte maturation factor 1, p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p35, IL-12 subunit p40, IL-12, subunit p35, IL-12A, IL-12B, IL12, subunit p40, IL35 subunit, interleukin 12, p40, interleukin 12A (natural killer cell stimulatory factor 1, p35), interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), interleukin-12 alpha chain, interleukin-12 beta chain, Interleukin-12 subunit alpha, Interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NK cell stimulatory factor chain 2, NKSF1, NKSF2
物种（Tested）	人
试剂盒类型	Proximity-based Immunoassay
标记（染料）	FAM
Gene ID	(Human) 3592,(Human) 3593
Gene aliases	CLMF, CLMF2, IL-12A, IL-12B, IL12A, IL12B, IMD28, IMD29, NFSK, NKSF, NKSF1, NKSF2, P35
Gene symbol	IL12A, IL12B
UniProt ID	(Human) P29459,(Human) P29460

The ProQuantum Human IL-12p70 Immunoassay Kit is designed to provide highly sensitive quantitative measurements of human IL-12p70 in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive Cloud-based software for analytics enables sample-to-answer in just 2 hours.ProQuantum人IL-12p70免疫测定试剂盒用于提供小样品量中人IL-12p70的高灵敏度定量测量。该分析利用基于邻近的扩增技术，将高亲和力抗体-抗原结合的分析物特异性与实时PCR的信号检测和扩增功能结合在一起，从而实现了一个简单而强大的下一代蛋白质定量平台。用户友好的工作流程与直观的基于云的软件相结合进行分析，可在2小时内完成从样品到答案的分析。ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology.ProQuantum免疫测定法使用一对配对的靶标特异性抗体，每种抗体均与DNA寡核苷酸偶联。在抗体-分析物结合过程中，两个DNA寡核苷酸紧密靠近，这允许两条链连接并随后产生用于扩增的模板链。该平台利用了Applied Biosystems TaqMan实时PCR技术的灵敏度和大动态范围。The assay workflow is fast and easy—2 steps in 2 hours. There are a total of 7 components in each kit (Figure 2). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum cloud-based software at https://apps.thermofisher.com/apps/proquantum. Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, set up customized assay instructions, and obtain robust statistical group-wise comparisons.测定工作流程快速简便— 2小时内完成2个步骤。每个试剂盒中总共有7个组份。首先，混合抗体-结合物，稀释样品，并在工作板上创建标准曲线。然后，使用多通道移液器，将抗体结合物和样品（或标准品）添加到PCR板的孔中，并孵育1小时。合并预混液和连接酶，并添加到PCR板的孔中，然后在任何qPCR仪器上运行该板。运行完成后，将结果文件导入https://apps.thermofisher.com/apps/proquantum的基于ProQuantum云的软件中。使用此软件，可以轻松分析数据以获得蛋白质浓度值。该软件允许您设置标准曲线，设计板布局，设置定制化验说明并获得可靠的逐组统计比较。Target information 靶标信息Interleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. IL-12 p70 is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Human and mouse IL-12 p40 share about 70% amino acid sequence homology.白介素12（IL-12）是一种异源二聚体70 kDa细胞因子，由两条共价连接的糖基化链组成，分子量分别为40kD（p40）和35-kD（p35）。 IL-12主要由单核细胞，巨噬细胞和树突状细胞响应细菌产品（如脂多糖（LPS）），细胞内病原体或与活化T细胞相互作用而产生。最初发现IL-12是因为它具有诱导干扰素-γ（IFN-g）产生，细胞增殖以及由自然杀伤细胞和T细胞介导的细胞毒性的能力。研究表明，IL-12在Th1反应的发展中也起着关键作用，导致IFN-g和IL-2的产生。这些细胞因子可进而促进T细胞反应和巨噬细胞活化。使用双启动子表达系统，在杆状病毒感染的昆虫细胞中产生重组小鼠IL-12 p70，作为前体p35和p40亚基的真正异源二聚体。 IL-12 p70与其他可用形式的蛋白质不同，因为它表达为真正的异源二聚体，而不是其中亚基通过人工接头连接的单链伪异源二聚体。淋巴细胞对此细胞因子的反应是由转录蛋白STAT4的激活剂介导的。发现一氧化氮合酶2A（NOS2A / NOS2）是先天免疫中该细胞因子信号传导过程所必需的。人和小鼠IL-12 p40共有约70％的氨基酸序列同源性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

特点：

- 高灵敏度 - 更高的灵敏度检测低水平的蛋白质- 宽动态范围- 样品消耗少 - 使用2–5 µL样品- 快速，轻松的工作流程 - 从样品到取样仅需2个小时，无需清洗- 无需购买专有仪器 - 可在任何实时PCR仪器上运行

数据：

IL-12p70 Human ProQuantum Immunoassay KitHow ProQuantum immunoassays work. (A) Antibody-antigen binding: Antibodies bind to two separate epitopes on the antigen (during a 1-hr incubation), which brings the two conjugated oligonucleotides into close proximity. (B) Ligation and amplification of signal (in a qPCR instrument): DNA ligase and a third splint oligonucleotide connector are added to ligate the two ends of the conjugated oligonucleotides, creating a 100-base DNA template. Once the ligase is inactivated at 95°C, the sample is amplified through 40 cycles of annealing and extension. The amount of DNA produced is measured after each amplification cycle via fluorescent dyes, which exhibit fluorescence that is directly proportional to the number of PCR product molecules (or amplicons) generated. This graph shows the fluorescence vs. cycle number curves for four different starting protein concentrations. The dashed line represents the fluorescence threshold. The cycle number required to reach the fluorescence threshold (threshold cycle or Ct) is plotted vs. protein concentration to create a standard curve.ProQuantum免疫测定如何工作。（A）抗体-抗原结合：抗体与抗原上的两个独立表位结合（在1小时的孵育过程中），这使两个结合的寡核苷酸紧密相邻。（B）信号的连接和扩增（在qPCR仪器中）：添加DNA连接酶和第三个夹板寡核苷酸连接物以连接缀合的寡核苷酸的两端，从而产生100个碱基的DNA模板。连接酶在95°C失活后，样品会通过40个退火和延伸循环进行扩增。在每个扩增循环后，通过荧光染料测量产生的DNA量，该荧光染料显示的荧光与产生的PCR产物分子（或扩增子）的数量成正比。该图显示了四种不同起始蛋白质浓度的荧光与循环数曲线。虚线表示荧光阈值。将达到荧光阈值所需的循环数（阈值循环或Ct）对蛋白质浓度作图，以创建标准曲线。

IL-12p70 Human ProQuantum Immunoassay Kit<b>ProQuantum kit contents</b><br />Components are color coded for ease of use.

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-45049.htm

特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系删除，本平台不提供信息存储服务。

Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.

产品询价需求提交

IL-12p70 Human ProQuantum Immunoassay Kit,IL-12p70 Human ProQuantum Immunoassay Kit/人IL-12p70 ProQuantum免疫测定试剂盒

相关推荐