IL-4 Human Matched Antibody Pair,IL-4 Human Matched Antibody Pair/人IL-4匹配抗体对

2024-10-23

IL-4 Human Matched Antibody Pair/人IL-4匹配抗体对

货号:CHC1283

规格:10 x 96 tests

价格:6895

产品类型:标准品和抗体对

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

15.6 pg/mL

检测范围15.6-1000 pg/mL

样本类型/体积

血清 100 μL

血浆 100 μL

上清液 100 μL

操作时间

1 hr 30 min

测试时间

24 hr

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 捕获(包被)抗体

- 检测抗体

- 标准品

- 链霉亲和素-HRP

储存温度

2-8℃

Protein nameIL-4
Protein aliasesB cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, Binetrakin, BSF-1, IL-4, interleukin 4 variant 2, Interleukin-4, Lymphocyte stimulatory factor 1, Pitrakinra

物种(Tested)

试剂盒类型

匹配抗体对

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesBCGF-1, BCGF1, BSF-1, BSF1, IL-4, IL4
Gene ID(Human) 3565
Gene symbolIL4
UniProt ID(Human) P05112
The Human IL-4 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-4. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationInterleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据:

IL-4 Human Matched Antibody PairRepresentative standard curve for Human IL-4 ELISA

技术参数
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