IL-2 Porcine Matched Antibody Pair,IL-2 Porcine Matched Antibody Pair/猪IL-2匹配抗体对

2024-10-23

IL-2 Porcine Matched Antibody Pair/猪IL-2匹配抗体对

货号:CSC1243

规格:10 x 96 tests

价格:6926

产品类型:标准品和抗体对

品牌:Thermo Fisher

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

35.6 pg/mL

检测范围35.6-570 pg/mL

样本类型/体积

血清 100 μL

血浆 100 μL

上清液 100 μL

操作时间

1 hr 30 min

测试时间

24 hr

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 捕获(包被)抗体

- 检测抗体

- 标准品

- 链霉亲和素-HRP

储存温度

2-8℃

Protein nameIL-2
Protein aliasesIL-2, T-cell growth factor, TCGF, Interleukin-2

物种(Tested)

试剂盒类型

匹配抗体对

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesIL-2, IL2, POIL2, TCGF
Gene ID(Pig) 396868
Gene symbolIL2
UniProt ID(Pig) P26891
The Porcine IL-2 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of porcine IL-2. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationInterleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据:

IL-2 Porcine Matched Antibody PairRepresentative standard curve for Porcine IL-2 ELISA

技术参数
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