IL-4 Porcine Matched Antibody Pair,IL-4 Porcine Matched Antibody Pair/猪IL-4匹配抗体对-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-4 Porcine Matched Antibody Pair,IL-4 Porcine Matched Antibody Pair/猪IL-4匹配抗体对

2024-10-23

IL-4 Porcine Matched Antibody Pair/猪IL-4匹配抗体对

货号：CSC1283

规格：10 x 96 Tests

价格：6791

产品类型：标准品和抗体对

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<15.6 pg/mL
检测范围	15.6-1000 pg/mL
样本类型/体积	血清 100 μL 血浆 100 μL 上清液 100 μL
操作时间	1 hr 30 min
测试时间	24 hr
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 捕获（包被）抗体 - 检测抗体 - 标准品 - 链霉亲和素-HRP
储存温度	2-8℃
Protein name	IL-4
Protein aliases	B-cell stimulatory factor 1, BSF-1, IL-4, Lymphocyte stimulatory factor 1, Interleukin-4
物种（Tested）	猪
试剂盒类型	匹配抗体对
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	IL4
Gene ID	(Pig) 397225
Gene symbol	IL4
UniProt ID	(Pig) Q04745

The Porcine Interleukin 4 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of pig IL-4. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationInterleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

技术参数

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IL-4 Porcine Matched Antibody Pair,IL-4 Porcine Matched Antibody Pair/猪IL-4匹配抗体对

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