Thermo Alexa Fluor 488 核酸标记,ARES Alexa Fluor 488 DNA Labeling Kit/Alexa Fluor 488 DNA标记试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo Alexa Fluor 488 核酸标记,ARES Alexa Fluor 488 DNA Labeling Kit/Alexa Fluor 488 DNA标记试剂盒

2024-10-24

ARES Alexa Fluor 488 DNA Labeling Kit/Alexa Fluor 488 DNA标记试剂盒

货号：A21665

规格：1 kit

价格：8364

产品类型：核酸标记

品牌：Thermo Fisher

The ARES Alexa Fluor DNA Labeling Kit provides a versatile, two-step method for labeling DNA with our Alexa Fluor dyes. In the first step, an amine-modified nucleotide is incorporated into DNA using conventional enzymatic labeling methods. In the second step, the amine-modified DNA is chemically labeled using our proprietary amine-reactive Alexa Fluor 488 dye. The labeled probes can be used for fluorescencein situhybridization (FISH) and microarray techniques. We offer ARES Alexa Fluor DNA Labeling Kits in five fluorescent colors, and each kit provides sufficient reagents for 5 to 10 labeling reactions of 1 to 5 µg DNA each.More Uniform Labeling With ARES Labeling KitsARES Alexa Fluor Labeling Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor dyes. This method achieves uniformity and consistency of labeling that is difficult to obtain with conventional enzymatic incorporation of labeled nucleotides.We also offer this two-step labeling technology in ourFISH Tag DNAandFISH Tag RNA Kits, which provide a complete workflow solution for FISH applications, including all of the reagents for probe synthesis, labeling, purification and an anti-fade reagent to help protect the signal during fluorescence microscopy.

数据：

Human metaphase chromosome spreads.Fluorescent probes generated with ARES DNA Labeling Kits hybridized to human metaphase chromosome spreads. Centromere probes specific for chromosomes 17, 1 and 15 were prepared by nick translation and labeled with kits containing green-fluorescent Alexa Fluor 488 (A21665), red-orange–fluorescent Alexa Fluor 546 (A21667) and red-fluorescent Alexa Fluor 594 (A21669) dyes. DNA was counterstained with the blue-fluorescent Hoechst 33342 dye (H1399, H3570, H21491), and the slides were mounted using the ProLong Antifade Kit (P7481). This multiple-exposure image was obtained with bandpass filter sets appropriate for fluorescein, rhodamine, Texas Red dye and DAPI.

A cDNA microarray hybridized with three different cDNA samples labeled using our ARES™ DNA Labeling Kits.Three-color microarray. A cDNA microarray was hybridized with three different cDNA samples that were labeled using the ARES™ Alexa Fluor® 488 DNA Labeling Kit (Cat. no. A21665), the ARES™ Alexa Fluor® 594 DNA Labeling Kit (Cat. no. A21669) and the ARES™ Alexa Fluor® 647 DNA Labeling Kit (Cat. no. A21676), respectively. Each sample was imaged separately using a PerkinElmer ScanArray 5000 microarray scanner, and the black-and-white images were pseudocolored so that green represents the Alexa Fluor® 488 dye, red represents the Alexa Fluor® 594 dye and blue represents the Alexa Fluor® 647 dye. The images were overlaid using an additive RGB color scheme to reveal spots that show differential expression. Where fluorescence from all fluorophores is equivalent, spots are white; where blue is low, spots are yellow; where green is low, spots are pink; and where red is low, spots are aqua. Only a portion of the microarray is shown. Image provided by Gerti Schut and Mike Adams, University of Georgia.

参考文献：

1.Cell cycle dependent morphology changes and associated mitochondrial DNA redistribution in mitochondria of human cell lines.2.Cold shock of a hyperthermophilic archaeon: Pyrococcus furiosus exhibits multiple responses to a suboptimal growth temperature with a key role for membrane-bound glycoproteins.3.DNA microarray analysis of the hyperthermophilic archaeon Pyrococcus furiosus: evidence for anNew type of sulfur-reducing enzyme complex.

技术参数

产品优点 • Dye (Ex/Em): Alexa Fluor® 488 (492/520 nm) • Achieves more uniform, consistent labeling than techniques for enzymatic incorporation of labeled nucleotides • Typically produces one dye per 12–20 bases • Optimal for FISH and microarrays

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Thermo Alexa Fluor 488 核酸标记,ARES Alexa Fluor 488 DNA Labeling Kit/Alexa Fluor 488 DNA标记试剂盒

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