IL-6 Monoclonal Antibody (MQ2-39C3), Biotin, eBioscience/IL-6单克隆抗体（MQ2-39C3），生物素，eBioscience

货号：13-7068-81,13-7068-85

规格：50 µg,500 µg

价格：1501,3489

产品类型：抗体和ELISA

品牌：eBioscience

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience
浓度：	0.5 mg/mL	用法：	0.5-2 µg/mL(ELISA)；Misc；Array

产品详细信息Description: The MQ2-39C3 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.Applications Reported: MQ2-39C3 has been reported for use in detection of human IL-6 by ELISA and immunoblotting (WB).Applications Tested: The MQ2-39C3 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-6 (IL-6) in combination with the purified MQ2-13A5 (14-7069) antibody for capture and recombinant human IL-6 (14-8069) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloidprogenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

数据

IL-6 Antibody (13-7068-81)PloS one 2016 -Fig 3 Multiplexed assay and antibody cross-reactivity testing. Fluorescent signal and binding curves for 5 different proteins (PSA, TNF-alpha, IL-6, IL-1beta, and GFP) quantitated in parallel on a single device. Concentrations for the primary and secondary antibodies used in this experiment were 600 nM and 6 nM, respectively. Each panel shows the signal obtained from the specific primary antibody to the given antigen detected with the various secondary fluorescently labeled detection antibodies. The results show that each antigen was specifically detected by the correct antibody pair and that no significant cross reactivity occurred between the antigens and antibodies tested in this experiment. Left y-axis: phycoerythrin labeled secondary antibody signal; right y-axis: Alexa Fluor 647 labeled secondary antibody signal (error bars are std. dev. n = 7). IL-6 Antibody (13-7068-81)PloS one 2016 -Published figure using IL-6 monoclonal antibody (Product # 13-7068-81) IL-6 Antibody (13-7068-81)The Journal of biological chemistry 2014 -FIGURE 5. TLR blocking experiments. 200,000 human PBMC cells were stimulated with GMMA from different strains. After a 4-h incubation, IL-6 release was measured by ELISA and plotted as average of duplicates with standard deviation. A, IL-6 release after stimulation with 0.0001-1000 ng/ml (10-fold steps) of GMMA from different strains. The rectangle highlights the concentration of GMMA used in blocking experiments. B-E, cells were incubated with 25 mug/ml anti-TLR4 ( dark red graphs ), 15 mug/ml anti-TLR2 ( green graphs ), or both ( violet graphs ) for 30 min before exposure to 1 or 10 ng of GMMA from Ss -p - OAg Delta msbB ( B ); Sf 2a -p - OAg Delta msbB ( C ); Ss -p - OAg Delta htrB ( D ); and Sf 2a -p - OAg Delta htrB ( E ). The graphs from experiments without blocking are shown in the same color as in A . A representative result of three independent experiments is shown.

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参考文献：

1.Disease markersMultiplatform Biomarker Discovery for Bladder Cancer Recurrence Diagnosis.2.PloS oneA microfluidic platform for high-throughput multiplexed protein quantitation.3.PloS oneA microfluidic platform for high-throughput multiplexed protein quantitation.4.CVIToll-Like Receptor Activation by Generalized Modules for Membrane Antigens from Lipid A Mutants of Salmonella enterica Serovars Typhimurium and Enteritidis.

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