IL-10 Monoclonal Antibody (JES5-2A5), Biotin, eBioscience/IL-10单克隆抗体（JES5-2A5），生物素，eBioscience

货号：13-7102-81,13-7102-85

规格：50 µg,500 µg

价格：1455,3821

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：其它

荧光染料：Biotin

类型:	单抗	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience
浓度：	0.5 mg/mL	用法：	Flow；0.5-2 µg/mL9ELISA)；Neu

产品详细信息Description: The JES5-2A5 antibody reacts with mouse interleukin-10 (IL-10). The JES5-2A5 antibody is a neutralizing antibody. Mouse IL-10 is an 18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.靶标信息Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.

数据

IL-10 Antibody (13-7102-81)Disease models and mechanisms 2016 -Published figure using IL-10 monoclonal antibody (Product # 13-7102-81) IL-10 Antibody (13-7102-81)PLoS pathogens 2014 -Figure 3 In vitro cytokine production from C. pneumoniae infected BMDM. BMDMs were prepared from C57BL/6 (B6) or B6.C3H- sst1 congenic mice, as described in the Methods. Cells were infected with C. pneumoniae at an MOI of 3:1, and supernatant harvested at 24 hpi for assay of the indicated cytokines. (A-D) In some cases, cells were pretreated with IFN-gamma for 30 min at the indicated concentrations prior to infection. (E-F) For antibody neutralization, cells were pretreated with indicated neutralizing antibody (anti-IL-10 or anti-IFN-beta) or isotype control at a concentration of 250 ng/ml prior to infection or IFN-gamma treatment (10 U/ml). Data is shown as the mean +- SEM from triplicate wells. Significance: NS, No significant difference; *, p<0.05; **, p<=0.01; ***, p<=0.001; ****, p<=0.0001. The results are representative of at least 3 independent experiments. IL-10 Antibody (13-7102-81)PLoS pathogens 2014 -Figure 6 Neutralization of IFN-beta protects cells from death and enhances C. pneumoniae replication. BMDMs prepared from C57BL/6 (B6) or B6.C3H- sst1 mice were infected with C. pneumoniae (MOI 3:1) in the presence or absence of IFN-gamma (5 U/ml). The ratio of dead cells to total cell number was calculated using propidium iodide and Hoechst staining, as described in the Methods section, and is reported as the percent cytotoxicity. Where indicated, antibody neutralization was carried out using neutralizing antibody against IL-10 or IFN-beta, or isotype control at a concentration of 250 ng/ml prior to infection or IFN-gamma treatment. A: Cytotoxicity was determined over time at 24, 48 and 72 hpi in Cp infected BMDM, and is reported as percent cell death. B: Effect of IL-10 and IFN-beta neutralization on Cp-induced cytotoxicity at 72 hpi. C: Effect of IL-10 and IFN-beta neutralization on Cp growth. Infected BMDM were lysed at 69 hpi, and recovered EBs were quantitatively cultured, as described in the Methods. All data shown above represents the mean +- SEM from triplicate wells, and is representative of at least two independent experiments. Significance: *, p<0.05; **, p<=0.01; ***, p<=0.001.

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参考文献：

1.Scientific reportsAnalysis of the Trichuris suis excretory/secretory proteins as a function of life cycle stage and their immunomodulatory properties.2.Nature communicationsNlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment.3.Mediators of inflammationNeisseria gonorrhoeae induces a tolerogenic phenotype in macrophages to modulate host immunity.4.Journal of immunology (Baltimore, Md. : 1950)Type I IFN receptor and the B cell antigen receptor regulate TLR7 responses via distinct molecular mechanisms.5.PloS oneOsteoclast activated FoxP3+ CD8+ T-cells suppress bone resorption in vitro.

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