TNF alpha Polyclonal Antibody, Biotin, eBioscience/TNF α 多克隆抗体，生物素，eBioscience

货号：13-7341-81,13-7341-85

规格：50 µg,500 µg

价格：1406,4074

产品类型：流式抗体

品牌：eBioscience

物种：人/小鼠/大鼠

宿主：其它

抗体亚型：IgG

荧光染料：Biotin

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	Flow；0.5-2 µg/mL（ELISA)

产品详细信息Description: The affinity purified polyclonal rabbit antibody reacts with mouse and rat tumor necrosis factor-alpha. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4 T cells, and NK cells. A 26 kDa form is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Bioactive TNF alpha exists as a homotrimer.Applications Reported: This polyclonal antibody has been reported for use in ELISA and ELISPOT.Applications Tested: The biotinylated rabbit anti-mouse/rat TNF alpha polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse or rat TNF alpha in combination with the purified anti-mouse/rat TNF alpha monoclonal antibody, clone TN3.19 (14-7423) for capture and recombinant mouse TNF alpha (14-8321) or rat TNF alpha (14-8321) as the standard. A suitable range of concentrations of this biotinylated antibody for ELISA detection is 0.5 - 2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL (mouse TNF alpha) or 5000 - 30 pg/mL (rat TNF alpha) should be included in each ELISA plate. Recognition of mouse TNF alpha by this assay is not interfered with by 1000x excess soluble TNF alpha receptor type I or II.Filtration: 0.2 µm post-manufacturing filtered.靶标信息TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

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参考文献：

1.CellOpposing Effects of Fasting Metabolism on Tissue Tolerance in Bacterial and Viral Inflammation.2.PloS oneInterferon-gamma and nitric oxide synthase 2 mediate the aggregation of resident adherent peritoneal exudate cells: implications for the host response to pathogens.3.PloS oneThe liver X receptor agonist GW3965 improves recovery from mild repetitive traumatic brain injury in mice partly through apolipoprotein E.HDAC inhibition p

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