TNF alpha Monoclonal Antibody (MAb11), Biotin, eBioscience/TNF α单克隆抗体（MAb11），生物素，eBioscience

货号：13-7349-81,13-7349-85

规格：50 µg,500 µg

价格：1455,3663

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：其它

荧光染料：Biotin

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience
浓度：	0.5 mg/mL	用法：	Flow；0.5-2 µg/mL（ELISA)；Neu；IHC；Misc

产品详细信息Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF-alpha ), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4^+ T cells. TNF-alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.Applications Reported: The MAb11 antibody has been reported for use indetection of human TNF alpha by ELISA.Applications Tested: The MAb11 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human TNF-alpha (TNF-a, TNFa) in combination with the purified MAb1 (14-7348) antibody for capture and recombinant human TNFa (14-8329) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.Filtration: 0.2 µm post-manufacturing filtered.靶标信息TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

数据

TNF alpha Antibody (13-7349-81)PloS one 2017 -Fig 3 Detection of GFP-expressing BCG by innate cells and association between absolute numbers of innate cells and mycobacterial growth inhibition. ( A ) Representative flow cytometry plot of IL-6, IL-12 and TNF-alpha cytokine expression by myeloid dentritic cells (mDCs), monocytes and neutrophils, measured in whole blood stimulated for 6 hours with BCG, BCG-GFP (shown) or LPS, relative to an unstimulated control sample. ( B ) Representative histograms indicating proportions of innate cells that phagocytosed BCG-GFP (green). ( C ) Absolute numbers of innate cell subsets per milliliter of unstimulated whole blood plotted against M . tb H37Rv growth. R and p values were calculated using Spearman and apos;s correlation. ( D ) Absolute numbers of BCG-GFP-positive mDCs, monocytes or neutrophils per mL of whole blood in adult individuals, stratified by QFT status. The inclusion of TruCount beads during the cell staining steps of the innate whole blood assay allowed determination of the absolute number of each subset of cells per mL of whole blood. The red and blue circles represent QFT+ and QFT- adults, respectively. Horizontal lines represent medians and whiskers, the interquartile range. Differences in absolute counts of BCG-GFP-positive innate cells between the groups were evaluated with the Mann-Whitney test (shown P values). The pie charts show relative proportions of BCG-GFP-positive cells among each innate cell subset. TNF alpha Antibody (13-7349-81)Cell 2017 -Figure 5 Predicted Neoepitopes with Higher Mutant than Wild-Type HLA-I Binding Affinity Elicit a T Cell Response (A) Representative scatterplots of TNF-alpha and IFN-gamma intracellular cytokine staining of CD8 + T cells after 21 days of culture with CEF peptides or DMSO as positive and negative controls or the predicted mutant peptides ( Figure S6 B). CEF = Cytomegalovirus, Epstein-Barr virus, Influenza virus. (B) Percentage of CD8 + T cells with double-positive intracellular staining (TNF-alpha and IFN-gamma) after incubation with each of the 43 predicted HLA-I neoepitopes, and HLA-I predicted binding affinity wild-type to mutant ratio ( Table S5 B). Mutation in gene FLG2 E1608K (P12) was found to be clonal after manual inspection in IGV ( Table S1 A).

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参考文献：

1.PloS oneApplication of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population.2.CellHeterogeneous Tumor-Immune Microenvironments among Differentially Growing Metastases in an Ovarian Cancer Patient.3.Immunologic researchThe distribution and function of human memory T cell subsets in lung cancer.4.FEMS immunology and medical microbiologyInduction of cytokines in a macrophage cell line by proteins of Clostridium difficile.

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