IL-17A Monoclonal Antibody (eBio17CK15A5), eBioscience/IL-17A单克隆抗体（eBio17CK15A5）

货号：14-7175-81,14-7175-85

规格：50 µg,500 µg

价格：1264,3395

产品类型：流式抗体

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBio17CK15A5

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	Flow；1-4 µg/mL（ELISA)

产品详细信息Description: The eBio17CK15A5 antibody reacts with mouse and rat IL-17A. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.Applications Reported: The eBio17CK15A5 has been reported useful for mouse IL-17A ELISA and ELISPOT capture.Applications Tested: The Affinity Purified eBio17CK15A5 antibody has been tested as the capture antibody in a sandwich ELISA for measurement of mouse IL-17A protein levels, in combination with the biotinylated eBio17B7 antibody (13-7177) for detection and recombinant mouse IL-17A (14-8171) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1.0 - 4.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

数据

IL-17A Antibody (14-7175-81)Journal of immunology (Baltimore, Md. : 1950) 2017 -Published figure using IL-17A monoclonal antibody (Product # 14-7175-81)

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流式抗体

参考文献：

1. Frontiers in immunologyIL-3 Is a Marker of Encephalitogenic T Cells, but Not Essential for CNS Autoimmunity.2. Journal of immunology (Baltimore, Md. : 1950)PRMT5-Selective Inhibitors Suppress Inflammatory T Cell Responses and Experimental Autoimmune Encephalomyelitis.3. Nature communicationsNlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment.4. Journal of immunology (Baltimore, Md. : 1950)PRMT5-Selective Inhibitors Suppress Inflammatory T Cell Responses and Experimental Autoimmune Encephalomyelitis.5. Journal of immunology (Baltimore, Md. : 1950)Lineage-Specific Metabolic Properties and Vulnerabilities of T Cells in the Demyelinating Central Nervous System.

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