iNOS Monoclonal Antibody (CXNFT), APC iNOS Monoclonal Antibody (CXNFT), APC Thermo-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

iNOS Monoclonal Antibody (CXNFT), APC iNOS Monoclonal Antibody (CXNFT), APC Thermo

2024-10-29

iNOS Monoclonal Antibody (CXNFT), APC

货号：17-5920-80,17-5920-82

规格：25μg,100μg

市场价格：1363,3360

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

荧光染料：Alexa Fluor 488

抗体类型	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), APC
浓度：	0.2 mg/mL	用法：	0.06 μg/test (Flow)；
产品详细信息Description: This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism. There are three isoforms of NOS that are encoded by three separate genes. NOS1 (neuronal NOS, nNOS) and NOS3 (endothelial NOS, eNOS) are constitutively expressed, while NOS2 is induced in response to bacterial endotoxins and inflammatory cytokines such as IFN gamma and TNF alpha. NOS2 is expressed by myeloid-derived suppressor cells and M1 macrophages but not alternatively activated M2 macrophages. NOS enzymes are functionally active only when they form homodimers, and dimerization of NOS2 occurs at steady-state concentrations of free Ca2+ such that NOS2 is functionally active when it is produced.Applications Reported: This CXNFT antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This CXNFT antibody has been tested by intracellular staining and flow cytometric analysis of LPS-stimulated mouse thioglycolate-elicited peritoneal exudate cells using the Intracellular Fixation and Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.
数据
iNOS Antibody (17-5920-82) in FlowMouse thioglycolate-elicited peritoneal exudate cells were stimulated overnight with LPS then surface stained with Anti-Mouse CD11b eFluor® 450 (Product # 48-0112-82) followed by fixation and permeabilization with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). The cells were then intracellularly stained with 0.03 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.03 µg of Anti-Mouse NOS2 APC (right). Total viable cells were used for analysis.
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参考文献：
1.Nature communicationsChloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype."Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry"2.Journal of cellular and molecular medicineEnhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells."Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry"3.The Journal of experimental medicineIrg1expression in myeloid cells prevents immunopathology duringM. tuberculosisinfection.AuthorsNair S,Huynh JP,Lampropoulou V,Loginicheva E,Esaulova E,Gounder AP,Boon ACM,Schwarzkopf EA,Bradstreet TR,Edelson BT,Artyomov MN,Stallings CL,Diamond MS4.Nature communicationsInducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses."Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry"5.Nature communicationsChloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype."Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry"

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