Zeba™ Spin Desalting Columns, 7K MWCO, 2 mL,Zeba™ Spin Desalting Columns, 7K MWCO, 2 mL/脱盐离心柱-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Zeba™ Spin Desalting Columns, 7K MWCO, 2 mL,Zeba™ Spin Desalting Columns, 7K MWCO, 2 mL/脱盐离心柱

2024-10-23

Zeba™ Spin Desalting Columns, 7K MWCO, 2 mL/脱盐离心柱

货号：89889,89890

规格：5 columns,25 columns

价格：817,4176

产品类型：蛋白提取/纯化

品牌：Thermo Fisher

Thermo Scientific Zeba 脱盐离心柱 (7K MWCO, 2mL) 是一种由专用高效尺寸排阻色谱树脂制备的聚丙烯产品，离心时可对蛋白进行高效脱盐和回收。Zeba 脱盐离心柱 (7K MWCO, 2 mL) 的特点:•高回收率—低结合树脂极大提高了蛋白回收率•快速—无需筛选或等待依赖重力流出的蛋白•易于使用—无需繁琐的色谱柱制备或平衡步骤•灵活性—可选用离心柱和过滤离心板，满足各种需求•经济实用—比其他市售树脂更经济实用稀释蛋白样品 (25 µ g/mL) 的回收率＞ 90%，可高效去除盐和其他小分子 (< 1000 MW)，同时回收蛋白和其他大分子 (> 7000 MW)。

可用形式：•离心柱—75 µL、0.5 mL（微量离心管）、2 mL、5 mL、10 mL 离心柱•96 孔离心板—预分配 96 孔过滤离心板，与手动或自动纯化的离心兼容。离心板可快速、一致地实现孔间和板间的重现性，适合小规模、高通量分离。

数据：

Protein recovery and desalting efficiencyProtein samples (1mg/mL) were prepared in 1.0M NaCl and 100µL samples were desalted using Zeba Spin Desalting Plates, 7K MWCO. Results were analyzed by BCA Protein Assay and conductivity measurements.

Better performance with Zeba Spin Desalting ColumnsThermo Scientific Zeba Columns provide higher protein recovery and less sample dilution over a wider range of sample concentrations and volume compared to alternative products. Zeba Spin Desalting Columns (7K MWCO, 10mL) and Disposable PD-10 Desalting Columns (#17-0851-01, GE Healthcare) were used to desalt 1.5, 2.5 and 3.5mL samples of bovine serum albumin (BSA) at concentrations of 0.04, 0.2, and 1mg/mL. Desalting was performed according to each manufacturer’s recommended protocols for either spin (centrifuge) or drip (gravity) procedures. Three sets of columns were equilibrated in final buffer and then loaded with 1.5, 2.5, and 3.5mL samples. For each electrophoresis gel, an aliquot of starting sample equal to 1µg of BSA was loaded in Lane 1 as the Load Control; all other desalted samples were loaded in the gel at the same volume as the Load Control. Differences in intensity between lanes are a combination of protein recovery and sample dilution caused by desalting.

技术参数

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Zeba™ Spin Desalting Columns, 7K MWCO, 2 mL,Zeba™ Spin Desalting Columns, 7K MWCO, 2 mL/脱盐离心柱

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