Zeba™ Spin Desalting Columns, 7K MWCO, 5 mL,Zeba™ Spin Desalting Columns, 7K MWCO, 5 mL/脱盐离心柱-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Zeba™ Spin Desalting Columns, 7K MWCO, 5 mL,Zeba™ Spin Desalting Columns, 7K MWCO, 5 mL/脱盐离心柱

2024-10-23

Zeba™ Spin Desalting Columns, 7K MWCO, 5 mL/脱盐离心柱

货号：89891,89892

规格：5 columns,25 columns

价格：1003,4433

产品类型：蛋白提取/纯化

品牌：Thermo Fisher

Thermo Scientific Zeba Spin Desalting Columns, 7K MWCO, 5 mL are polypropylene devices containing a proprietary high-performance size-exclusion chromatography resin that provides an excellent protein desalting and recovery in a centrifuge format.Features of Zeba Spin Desalting Columns, 7K MWCO, 5 mL:•High recovery—low-binding resin maximizes protein recovery•Fast—no fraction screening or waiting for protein to emerge by gravity-flow•Easy-to-use—no cumbersome column preparation or equilibration•Flexible—available in spin columns, filter spin plates and cartridges for a range of needs•Economical—great performance at lower cost than other commercially available cartridgesEven very dilute (25 µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (< 1000 MW) and good recovery of proteins and other macromolecules (> 7000 MW).Available Formats:•Spin Columns—75 µL, 0.5 mL (microcentrifuge), 2 mL, 5 mL, 10 mL columns•Chromatography Cartridges—compatible with automated liquid chromatography systems and with syringe processing (learn more about our Chromatography Cartridges)•96-well Spin Plates—pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations.

数据：

Protein recovery and desalting efficiencyProtein samples (1mg/mL) were prepared in 1.0M NaCl and 100µL samples were desalted using Zeba Spin Desalting Plates, 7K MWCO. Results were analyzed by BCA Protein Assay and conductivity measurements.

Better performance with Zeba Spin Desalting ColumnsThermo Scientific Zeba Columns provide higher protein recovery and less sample dilution over a wider range of sample concentrations and volume compared to alternative products. Zeba Spin Desalting Columns (7K MWCO, 10mL) and Disposable PD-10 Desalting Columns (#17-0851-01, GE Healthcare) were used to desalt 1.5, 2.5 and 3.5mL samples of bovine serum albumin (BSA) at concentrations of 0.04, 0.2, and 1mg/mL. Desalting was performed according to each manufacturer’s recommended protocols for either spin (centrifuge) or drip (gravity) procedures. Three sets of columns were equilibrated in final buffer and then loaded with 1.5, 2.5, and 3.5mL samples. For each electrophoresis gel, an aliquot of starting sample equal to 1µg of BSA was loaded in Lane 1 as the Load Control; all other desalted samples were loaded in the gel at the same volume as the Load Control. Differences in intensity between lanes are a combination of protein recovery and sample dilution caused by desalting.

技术参数

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Zeba™ Spin Desalting Columns, 7K MWCO, 5 mL,Zeba™ Spin Desalting Columns, 7K MWCO, 5 mL/脱盐离心柱

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