ICAM-2 (Soluble) Human ELISA Kit ELISA试剂盒,ICAM-2 (Soluble) Human ELISA Kit/人ICAM-2(可溶)ELISA试剂盒

2024-10-23

ICAM-2 (Soluble) Human ELISA Kit/人ICAM-2(可溶)ELISA试剂盒

货号:BMS236

规格:96 tests

价格:1

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.30 U/mL

检测范围0.75-24.0 U/mL

样本类型/体积

羊水 100 µL

血浆 15 µL

血清 15 µL

上清液 100 µL

尿液 100 µL

全血 100 µL

操作时间

1 hr 20 min

测试时间

1 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)7%
批内变异系数(CV)6.7%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 对照

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameICAM-2
Protein aliasesCD102, ICAM-2, Intercellular adhesion molecule 2

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCD102, ICAM2
Gene ID(Human) 3384
UniProt ID(Human) P13598
Gene symbolICAM2

The Human Intercellular Adhesion Molecule-2(Hu ICAM-2) ELISA quantitates Hu ICAM-2 in human serum, plasma, amniotic fluid, urine, whole blood, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ICAM-2.人细胞间黏附分子2(人ICAM-2)ELISA定量测定了人类血清,血浆,羊水,尿液,全血或细胞培养基中的人ICAM-2。 该检测试剂盒将专门识别天然和重组人ICAM-2。Principle of the method 方法原理The Human ICAM-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人ICAM-2固相夹心ELISA(酶联免疫吸附测定)旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息ICAM-2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM-2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. Diseases associated with ICAM2 include Colon Carcinoma In Situ.ICAM-2是细胞间粘附分子(ICAM)家族的成员。 所有ICAM蛋白均为I型跨膜糖蛋白,包含2-9个免疫球蛋白样C2型结构域,并与白细胞粘附LFA-1蛋白结合。 ICAM-2可能通过阻断LFA-1依赖性细胞粘附而在淋巴细胞再循环中发挥作用。 它介导对抗原特异性免疫反应,NK细胞介导的清除,淋巴细胞再循环以及对免疫反应和监视重要的其他细胞相互作用重要的粘附相互作用。 已发现该基因的几种编码相同蛋白质的转录物变体。 与ICAM2有关的疾病包括原位结肠癌。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

ICAM-2 (Soluble) Human ELISA KitRepresentative standard curve for Human ICAM-2 ELISA

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