ICAM-1 (Soluble) Human ELISA Kit ELISA试剂盒,ICAM-1 (Soluble) Human ELISA Kit/人ICAM-1（可溶）ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

ICAM-1 (Soluble) Human ELISA Kit ELISA试剂盒,ICAM-1 (Soluble) Human ELISA Kit/人ICAM-1（可溶）ELISA试剂盒

2024-10-23

ICAM-1 (Soluble) Human ELISA Kit/人ICAM-1（可溶）ELISA试剂盒

货号：BMS201,BMS201TEN

规格：96 Tests,10X96 Tests

价格：1,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	2.2 ng/mL
检测范围	6.25-100 ng/mL
样本类型/体积	血清 10 μL 血浆 10 μL 上清液 10 μL
操作时间	1 hr 20 min
测试时间	1 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	7.7%
批内变异系数（CV）	4.1%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 -检测抗体偶联物：抗兔HRP - 洗涤液 - 对照 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	ICAM-1 (CD54)
Protein aliases	CD54, cell surface glycoprotein P3.58, ICAM-1, Major group rhinovirus receptor, Intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	BB2, CD54, ICAM1, P3.58
Gene ID	(Human) 3383
UniProt ID	(Human) P05362
Gene symbol	ICAM1

The Human Soluble Intercellular Adhesion Molecule 1(Hu sICAM-1) ELISA quantitates Hu sICAM-1 in human serum, plasma, cell culture supernatants, urine, amniotic fluid, bile, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sICAM-1.人可溶性细胞间粘附分子1（人sICAM-1）ELISA定量分析了人类血清，血浆，细胞培养上清液，尿液，羊水，胆汁或其他体液中的人sICAM-1。该检测试剂盒将专门识别天然和重组人sICAM-1。Principle of the method 方法原理The Human sICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人sICAM-1固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。通过将第二（检测）抗体与与捕获抗体不同的表位上的靶标结合形成三明治。与酶缀合的抗体结合形成的三明治。经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.ICAM-1（CD54）是一种85-110 kDa的单链1型整合膜糖蛋白，具有五个免疫球蛋白超家族重复序列，一个跨膜区和一个胞质区的胞外域。 ICAM-1具有7个潜在的N-连接糖基化位点，并与ICAM-3（CD50）和ICAM-2（CD102）具有相当的氨基酸序列同源性。 ICAM-1与CD11a / CD18（白细胞粘附分子，LFA-1）或CD11b / CD18（Mac-1）类型的整联蛋白结合，并被鼻病毒用作受体。 ICAM-1由活化的内皮细胞表达，并在上皮细胞，成纤维细胞，软骨细胞，B淋巴细胞，T淋巴细胞（低），单核细胞，巨噬细胞，树突状细胞和嗜中性粒细胞中检测到，其水平随着炎症而增加。在某些癌细胞和黑色素瘤细胞中也检测到ICAM-1。在血浆中可检测到可溶性ICAM-1，而在患有各种炎症综合症的患者中则升高。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

ICAM-1 (Soluble) Human ELISA KitRepresentative standard curve for Human ICAM-1 ELISA

技术参数

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ICAM-1 (Soluble) Human ELISA Kit ELISA试剂盒,ICAM-1 (Soluble) Human ELISA Kit/人ICAM-1（可溶）ELISA试剂盒

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