IL-15/IL-15R Complex Mouse ELISA Kit ELISA试剂盒,IL-15/IL-15R Complex Mouse ELISA Kit/复合小鼠IL-15 / IL-15R ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-15/IL-15R Complex Mouse ELISA Kit ELISA试剂盒,IL-15/IL-15R Complex Mouse ELISA Kit/复合小鼠IL-15 / IL-15R ELISA试剂盒

2024-10-23

IL-15/IL-15R Complex Mouse ELISA Kit/复合小鼠IL-15 / IL-15R ELISA试剂盒

货号：BMS6023,BMS6023TEN

规格：96 Tests,10X96 Tests

价格：1,34832

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	2.0 pg/mL
检测范围	6.3-400 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	6.3%
批内变异系数（CV）	5.3%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-15/IL-15R
Protein aliases	CD215, IL-15, IL-15 receptor subunit alpha, IL-15R-alpha, Interleukin-15, Interleukin-15 receptor subunit alpha, sIL-15 receptor subunit alpha, Soluble interleukin-15 receptor subunit alpha
物种（Tested）	小鼠
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	AA690181, AI503618, IL-15, IL-15RA, Il15, Il15ra
Gene ID	(Mouse) 16168,(Mouse) 16169
Gene symbol	Il15, Il15ra
UniProt ID	(Mouse) P48346,(Mouse) Q60819

The Mouse Interleukin-15/Interleukin-15 Receptor Complex (Ms IL-15/15R) ELISA quantitates Ms IL-15/15R in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-15/15R.小鼠白细胞介素15 /白细胞介素15受体复合物（小鼠IL-15 / 15R）ELISA定量分析了小鼠血清，血浆，缓冲液或细胞培养基中的小鼠IL-15 / 15R。该检测试剂盒将专门识别天然和重组小鼠IL-15 / 15R。Principle of the method 方法原理The Mouse IL-15/15R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠IL-15 / 15R固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.白介素15（IL-15）是一种14 kDa的促炎蛋白，已被证明在嗜中性粒细胞，树突状细胞和巨噬细胞的活化中起作用，并且对NK细胞和CD8 T细胞的发育和存活至关重要。 IL-15通过异源三聚体受体发出信号来激活Jak / Stat途径。该受体的两个亚基β和γ与IL-2受体共有，而α亚基是IL-15所特有的。尽管IL-15 mRNA在许多细胞类型中都有表达，但分泌的蛋白很少在生物样品中可检测到。最近的研究表明，IL-15保留在细胞内，仅以其独特的受体IL-15Rα的复合物形式分泌。从ER到高尔基体直到分泌都需要这种陪伴。细胞因子/受体复合物可以保持与细胞表面的结合，在这里它可以被转递到表达受体β/γ亚基的细胞上，或者可以以缺乏受体跨膜结构域的可溶形式分泌。可溶性IL-15 / IL-15R复合物是由小鼠树突状细胞，成纤维细胞和巨噬细胞产生的，与单独的IL-15相比，其激动活性提高了10-100倍。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-15/IL-15R Complex Mouse ELISA KitRepresentative standard curve for Mouse IL-15 Receptor ELISA

技术参数

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