IL-17A Human ELISA Kit,IL-17A Human ELISA Kit/人IL-17A ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-17A Human ELISA Kit,IL-17A Human ELISA Kit/人IL-17A ELISA试剂盒

2024-10-23

IL-17A Human ELISA Kit/人IL-17A ELISA试剂盒

货号：BMS2017,BMS2017TEN

规格：96 Tests,10X96 Tests

价格：1,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.5 pg/mL
检测范围	1.6-100 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	9.1%
批内变异系数（CV）	7.1%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 对照 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-17A
Protein aliases	CTLA-8, Cytotoxic T-lymphocyte-associated antigen 8, cytotoxic T-lymphocyte-associated protein 8, IL-17, IL-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), Interleukin-17A
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CTLA-8, CTLA8, IL-17, IL-17A, IL17, IL17A
Gene ID	(Human) 3605
Gene symbol	IL17A
UniProt ID	(Human) Q16552

The Human Interleukin-17A (Hu IL-17A) ELISA quantitates Hu IL-17A in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-17A.人白介素17A（人IL-17A）ELISA定量了人血清，血浆，缓冲液或细胞培养基中的人IL-17A。该检测试剂盒将只识别天然和重组的人IL-17A。Principle of the method 方法原理The Human IL-17A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-17A固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).白介素-17A（IL-17A，CTLA-8）是CD4 + T细胞衍生的细胞因子，可促进细胞系中的炎症反应，并在类风湿性关节炎，哮喘，多发性硬化症，牛皮癣和移植排斥反应中升高。 IL-17A是一个32 kDa长的二硫键连接的同型二聚体，由136个氨基酸组成，是六种蛋白质家族（IL-17A-17F）的成员，并通过IL-17受体（IL-17R / CDw217）。活化的外周血CD4 + T细胞产生高水平的IL-17A同型二聚体，并且IL-17A还增强人成纤维细胞中细胞内粘附分子1（ICAM-1）的表达。特别地，人IL-17A还刺激上皮，内皮或成纤维细胞分泌IL-6，IL-8，G-CSF和PGE2。在人IL-17A存在的情况下，成纤维细胞可以维持CD34 +造血祖细胞的增殖并诱导成熟为嗜中性粒细胞。小鼠，大鼠和人IL-17A可以诱导小鼠基质细胞分泌IL-6，这表明所有同源物都可以识别小鼠受体。 IL-17A调节NF-κB和丝裂原激活的蛋白激酶的活性，刺激IL-6和环氧合酶2（PTGS2 / COX-2）的表达，并增强一氧化氮（NO）的产生。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-17A Human ELISA KitRepresentative standard curve for Human IL-17A ELISA

技术参数

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IL-17A Human ELISA Kit,IL-17A Human ELISA Kit/人IL-17A ELISA试剂盒

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