IL-27 Human ELISA Kit ELISA试剂盒,IL-27 Human ELISA Kit/人IL-27 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-27 Human ELISA Kit ELISA试剂盒,IL-27 Human ELISA Kit/人IL-27 ELISA试剂盒

2024-10-23

IL-27 Human ELISA Kit/人IL-27 ELISA试剂盒

货号：BMS2085,BMS2085TEN

规格：96 Tests,10X96 Tests

价格：1,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	9.5 pg/mL
检测范围	62.5-4,000 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	5.6%
批内变异系数（CV）	8.5%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-27 p28
Protein aliases	IL-27 p28 subunit, IL-27 subunit alpha, IL-27-A, IL27-A, interleukin 30, Interleukin-27 subunit alpha, Interleukin-30, p28
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	IL-27, IL-27A, IL27, IL27A, IL27p28, IL30, p28
Gene ID	(Human) 246778
Gene symbol	IL27
UniProt ID	(Human) Q8NEV9

The Human Interleukin-27 (Hu IL-27) ELISA quantitates Hu IL-27 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-27.人白介素27（人IL-27）ELISA定量了人血清，血浆，缓冲液或细胞培养基中的人IL-27。该检测试剂盒将只识别天然和重组的人IL-27。Principle of the method 方法原理The Human IL-27 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-27固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30). IL-27 is produced by activated APCs and mature dendritic cells. IL-27 exerts its activities on NK cells and naive CD4+ T cells; mRNA expression analysis of IL-27 receptor components (WSX-1/TCCR and gp130) suggests that IL-27 may also target other cells, including mast cells and monocytes. Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 and STAT1/3 phosphorylation. WSX-1/TCCR-deficient mice develop impaired Th1 responses and are more susceptible to infection with L. monocytogenes suggesting that Th1 responses require IL-27. Although activation of WSX-1 is required for the initiation of Th1 responses, it is not necessary for maintaining Th1 responses. IL-27 alone is not able to induce the differentiation of CD4+ T cells into IFN-γ-producing cells, suggesting a role for IL-27 as an initial activator of Th1 responses. An important effect of IL-27 in initiating Th1 responses is the induction of the Th1-specific transcription factor T-bet as well as the suppression of the Th2-specific transcription factor GATA-3. T-bet plays a critical role in Th1 differentiation by its ability to maintain IL-12Rβ2 expression following CD4+ T cell activation. Recent studies indicate that IL-27 has a potent antitumor activity. In vitro, IL-27 has been found to act directly on naive CD8 cells, generating CTL with enhanced granzyme B expression. In vivo, IL-27 has been reported to augment CTL activity, inhibit tumor growth, and induce complete regression of primary and metastatic neuroblastoma tumors.IL-27是IL-12家族的成员，是一种异二聚体蛋白，由p40相关蛋白爱泼斯坦-巴尔病毒诱导的基因3（EBI3）非共价连接到IL-12p35相关蛋白p28（也称为IL-30）。 IL-27由活化的APC和成熟的树突状细胞产生。 IL-27在NK细胞和天然CD4 + T细胞上发挥活性。 IL-27受体成分（WSX-1 / TCCR和gp130）的mRNA表达分析表明，IL-27也可能靶向其他细胞，包括肥大细胞和单核细胞。 IL-27与WSX-1 / gp130的结合可激活JAK1，STAT1和STAT3和STAT1 / 3磷酸化。 WSX-1 / TCCR缺陷型小鼠的Th1反应受损，并且更容易感染单核细胞增生李斯特菌，提示Th1反应需要IL-27。尽管启动Th1响应需要激活WSX-1，但并不需要维持Th1响应。单独的IL-27不能诱导CD4 + T细胞分化为产生IFN-γ的细胞，提示IL-27作为Th1应答的初始激活物。 IL-27在启动Th1反应中的重要作用是诱导Th1特异性转录因子T-bet以及抑制Th2特异性转录因子GATA-3。 T-bet通过在CD4 + T细胞活化后维持IL-12Rβ2表达的能力，在Th1分化中起关键作用。最近的研究表明IL-27具有有效的抗肿瘤活性。在体外，已经发现IL-27直接作用于幼稚CD8细胞，产生具有增强的粒酶B表达的CTL。在体内，据报道IL-27增强CTL活性，抑制肿瘤生长，并诱导原发性和转移性神经母细胞瘤肿瘤完全消退。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-27 Human ELISA KitRepresentative standard curve for Human IL-27 ELISA

技术参数

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IL-27 Human ELISA Kit ELISA试剂盒,IL-27 Human ELISA Kit/人IL-27 ELISA试剂盒

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