PMN (Neutrophil) Elastase Human ELISA Kit ELISA试剂盒,PMN (Neutrophil) Elastase Human ELISA Kit/人PMN（中性粒细胞）弹性蛋白酶ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

PMN (Neutrophil) Elastase Human ELISA Kit ELISA试剂盒,PMN (Neutrophil) Elastase Human ELISA Kit/人PMN（中性粒细胞）弹性蛋白酶ELISA试剂盒

2024-10-23

PMN (Neutrophil) Elastase Human ELISA Kit/人PMN（中性粒细胞）弹性蛋白酶ELISA试剂盒

货号：BMS269

规格：96 Tests

价格：1

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	1.98 pg/mL
检测范围	0.16-10.0 ng/mL
样本类型/体积	血浆 10 μL 脑脊液 10 μL 灌洗液 10 μL 上清液 10 μL
操作时间	1 hr 20 min
测试时间	2 hr 20 min
Homogenous (no wash)	No
批间变异系数（CV）	5.6%
批内变异系数（CV）	4.8%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - HRP偶联检测抗体 - 洗涤液 - 对照 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	Neutrophil elastase
Protein aliases	Bone marrow serine protease, elastase 2, neutrophil, Elastase-2, granulocyte-derived elastase, HLE, Human leukocyte elastase, leukocyte elastase, Medullasin, Neutrophil elastase, PMN elastase, polymorphonuclear elastase
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	ELA2, ELANE, GE, HLE, HNE, NE, PMN-E, SCN1
Gene ID	(Human) 1991
Gene symbol	ELANE
UniProt ID	(Human) P08246

The Human PMN (Neutrophil) Elastase) ELISA quantitates Hu PMN (Neutrophil) Elastase in human plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant PMN (Neutrophil) Elastase.人PMN（中性粒细胞）弹性蛋白酶ELISA定量测定人血浆，渗出液，支气管肺泡灌洗液，脑脊液和精浆或细胞培养基中的Hu PMN（中性粒细胞）弹性蛋白酶。该测定法将专门识别天然和重组PMN（中性粒细胞）弹性蛋白酶。Principle of the method 方法原理The Human PMN (Neutrophil) Elastase solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人PMN（嗜中性粒细胞）弹性蛋白酶固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.弹性蛋白酶的中性粒细胞形式长218个氨基酸，带有两条与天冬酰胺连接的碳水化合物链（请参见糖基化）。它存在于嗜中性粒细胞胞质中的嗜蓝粒颗粒中。中性粒细胞弹性蛋白酶似乎有两种形式，称为IIa和IIb。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

PMN (Neutrophil) Elastase Human ELISA KitRepresentative standard curve for PMN Elastase ELISA

技术参数

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PMN (Neutrophil) Elastase Human ELISA Kit ELISA试剂盒,PMN (Neutrophil) Elastase Human ELISA Kit/人PMN（中性粒细胞）弹性蛋白酶ELISA试剂盒

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